在2-氯TRT树脂上挂个FMOC氨基酸的方法

上海信裕生物科技有限公司

2017/12/23 16:38
TA的动态

在2-氯TRT树脂上挂个FMOC氨基酸的方法

  • Weigh 10 g 2-chlorotrityl chloride resin (15 mmol) in a reaction vessel, wash with DMF (2x), swell the resin in 50 mL DMF for 10 min, drain vessel.

  • Weigh 10 mmol Fmoc-amino acid in a test tube, dissolve Fmoc-amino acid in 40 mL DMF, transfer the solution into the reaction vessel above, add 8.7 mL DIEA (50 mmol), swirl mixture for 30 min at room temperature.

  • Add 5 mL methanol into the reaction vessel and swirl for 5 min.

  • Drain and wash with DMF (5x).

  • Check substitution.

  • Add 50 mL 20% piperidine to remove the Fmoc group. Swirl mixture for 30 min.

  • Wash with DMF (5x), DCM (2x), put resin on tissue paper over a foam pad and let dry at room temperature overnight under the hood. Cover the resin with another piece of tissue paper, press lightly to break aggregates.

  • Weigh loaded resin.

  • Pack in appropriate container.


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