供货周期: | 现货 |
品牌: | AMRESCO |
型号: | g/ml |
货号: |
用途:快速硫化氢试验琼脂用于分子生物学中的培养
原理:蛋白胨提供碳源和氮源;葡萄糖提供能源;琼脂是培养基的凝固剂。
用法:称取本品65.0g,加热搅拌溶解于1000ml蒸馏水中121℃高压灭菌15分钟,冷却至50℃左右时,加入过滤除菌的氯霉素50mg,混匀,倾入无菌平皿。
【注意事项】:由于液体培养基不易长期保管,现在均改制成粉末。
培养基有天然、人工两种快速硫化氢试验琼脂一般实验室使用的是RPMI-1640粉末培养基,以双蒸或三蒸水配制。NaHCO3(或HCl)调pH至7.2~7.4,若pH值超过7.6或远低于6.8,则大多数细胞不能生长。RPMI-1640不耐高压灭菌,使用前需经灭菌0.22μm孔径滤膜滤过处理。
快速硫化氢试验琼脂的保存
1)干粉,买了后一定要保存在4度。有人保存在-20度我认为没有必要。但4度是非常必要的。保证期是有提示的。
2)配好的无血清培养基一定保存在4度,保存不要超过3个月。用时根据提示要加入谷胺酰胺。
3)加入血清的培养基,最好保存在4度不要超过1个月,从时间太长,活性下降。当然稍微长点也不一定出问题。但是根据细胞而定;如果原代培养,最好新鲜,如果是肿瘤细胞,或仅维持传代就不太要紧。但是原则是越新越好。
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A-01186 ?Anti-ATRN(attractin)
A-01187 ?Anti-ATP7B
A-01188 ?Anti-ATP1b2/Na+K+ ATPase(ATPase, Na+/K+ transporting, beta 2 polypeptide)
A-01189 ?Anti-AVEN(apoptosis, caspase activation inhibitor)
A-01190 ?Anti-ATP-sensitive K+ channel subunit Kir6.2
A-01191 ?Anti-AVPR2(arginine vasopressin receptor 2)
A-01192 ?Anti-Bdkrb2/B2R(Bradykinin receptor beta 2)
A-01193 ?Anti-Axin1(Axis inhibition protein 1)
A-01194 ?Anei-ATX/Autotaxin/E-NPP2(Ectonucleotide pyrophosphatase/phosphodiesterase family member 2)
A-01195 ?Anti-Aurora B
A-01196 ?Anti-B7-H1/PDL1/CD274(programmed death ligand 1)
A-01197 ?Anti-B7-DC/PDL2/CD273(programmed death ligand 2)
A-01198 ?Anti-B7H4 ( V-set domain containing T cell activation inhibitor 1 )
A-01199 ?Anti-BACE/ASP2
A-01200 ?Anti-Bad (BCL-xL/BCL-2-associated death promoter)
A-01201 ?Anti-Bad (BCL-xL/BCL-2-associated death promoter)
A-01202 ?Anti-phospho-BAD(pSer128)
A-01203 ?Anti-Bak(BCL2 homologous antagonist/killer)
A-01204 ?Anti-BADH2 (Betaine-aldehyde dehydrogenase )
A-01205 ?Anti-Bassoon/BSN(zinc finger protein 231)
A-01206 ?Anti-Batroxobin
A-01207 ?Anti-Bax (Bcl-2 Assaciated X protein)
A-01208 ?Anti-Bax(Bcl-2 Assaciated X protein)
A-01209 ?Anti-Bcl-2(B cell lymphoma/leukemia-2)
A-01210 ?Anti-Bcl-xL
A-01211 ?Anti-BBC3/PUMA(bcl-2 binding component 3)
A-01212 ?Anti-phospho-Bcl-xL(pSer62)
A-01213 ?Anti-BCHE(butyrylcholinesterase)NT
A-01214 ?Anti-BCHE(mouse Anti-butyrylcholinesterase Monoclonal)
A-01215 ?Anti-BCHE(butyrylcholinesterase)CT
A-01216 ?Anti-BDNF (Brain-derived Neurotrophin factor)
A-01217 ?Anti-BECN1(Beclin 1)
A-01218 ?Anti-Beta-casein(β-casein)
A-01219 ?Anti-BAFF/CD257(B cell activating factor)
A-01220 ?Anti-BFGF/FGF-2
A-01221 ?Anti-BGP(Osteocalcin/Bone Gla-protein)
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Recombinant Tumor Protein, Translationally Controlled 1 (TPT1) XY95191RPMu01
Recombinant Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) XY91659RPHu01
Recombinant Mitochondrial Uncoupling Protein 2 (UCP2) XY93249RPRa01
Recombinant Peroxisome Proliferator Activated Receptor Gamma (PPARg) XY93786RPBo01
Recombinant Glycogen Phosphorylase, Muscle (PYGM) XY92226RPMu01
Recombinant Macrophage Inflammatory Protein 3 Alpha (MIP3a) XY93067RPMu01
Recombinant Monocyte Chemotactic Protein 3 (MCP3) XY93281RPRa01
Cell Counting Kit-8(CCK-8)细胞增殖检测试剂盒
FITC-Annexin V/PI 细胞凋亡试剂盒
1,5-AG(1,5-Anhydroglucitol) ELISA Kit XY9U2590
Recombinant Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Theta (YWHAq) XY95200RPHu01
Recombinant Spindlin 1 (SPIN1) XY94760RPHu
OVA Conjugated Leucine (Leu) XY95654CPSGe
Recombinant Peroxiredoxin 5 (PRDX5) XY93776RPMu02
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