供货周期: | 现货 |
品牌: | 信裕生物 |
规格: | 10ug/50ug/200ug/1mg |
货号: | XY91025RPBo01 |
CAS号: |
Recombinant Chromogranin B (CHGB)
嗜铬蛋白B(CHGB)重组蛋白
[ PROPERTIES ]
Residues: Leu309~Glu484,with two N-terminal Tags, His-tag and GST-tag.
Host: E. coli
Subcellular Location: Cytoplasmic vesicle,secretory vesicle membrane; Peripheralmembrane protein; Lumenal side.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 5.9
Predicted Molecular Mass: 51.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37ºC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -LE EGAEYGEEVRRHSAAQAPGD LQGA RFGGRG RGE HQA LRRP SEES LEQE NK RHG LSP D L NMAQ GY S EE S EE ER GPA PGP S Y RA R G GEA A AY ST LG Q T DE K R FLGE T H H RVQESQRDKA RRR LPGE LRNY LD YGEEKGEEAA RGKWQPQGDP RDA DE NREEARLRGKQYAPH HITE
[ IDENTIFICATION ]
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!以实际收货产品说明书为准,网站说明书仅供参考!
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