供货周期: | 现货 |
品牌: | Sigma-Aldrich |
规格: | AR级别,99% |
货号: | N128634 |
CAS号: |
Product Name: Nuclease S1 from Aspergillus oryzae
CAS号: | 37288-25-8 |
MDL号: | MFCD00131010 |
介绍: | Nuclease S1 isolated from certain Neurospora and Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. Nuclease S1 has a molecular weight of approximately 34 kDa and exists as a monomer. The optimum pH range is 4.0-4.6, and it is activated by Zn2+ and/or Ca2+. Inhibitors are EDTA, citrate and high concentrations of SDS. |
用途: | Nuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA.It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor. |
贮存: | 储存温度 -20°C |
生化和生理学机理: | Nuclease S1 isolated from Aspergillus oryzae exhibits endo- and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5’-phosphomononucleotide and 5’-phosphooligonucleotide end-products. It is used to digest non-annealed polynucleotide tails and hairpin loops in RNA and DNA duplexes and can be used to convert superhelical DNA to the linear form. |
相关产品
GelstainRedTM 核酸染料, 10,000× in water
Super GelBlueTM 核酸染料, 10,000× in water
彩色预染蛋白Marker(10-180 kDa,三色)
彩色预染蛋白Marker(10-250kDa, 双色)
琼脂糖 A2015
Super ECL Plus(超敏化学发光检测试剂盒) S6009M
PAGE 彩色快速凝胶制备试剂盒(10%)
Minerva Super Fusion Cloning Kit(无缝克隆试剂盒)
Cell Counting Kit-8(CCK-8)细胞增殖检测试剂盒
FITC-Annexin V/PI 细胞凋亡试剂盒
1,5-AG(1,5-Anhydroglucitol) ELISA Kit XY9U2590
Recombinant Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Theta (YWHAq) XY95200RPHu01
Recombinant Spindlin 1 (SPIN1) XY94760RPHu
OVA Conjugated Leucine (Leu) XY95654CPSGe
Recombinant Peroxiredoxin 5 (PRDX5) XY93776RPMu02
关注
拨打电话
留言咨询