氧核糖核酸酶Ⅱ 来源于猪脾(纯化)

参考价：¥1
供货周期： 现货
品牌： Sigma-Aldrich
规格： AR级别，99%
货号： D128602
CAS号：
上海信裕生物科技有限公司
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D128602

氧核糖核酸酶Ⅱ 来源于猪脾(纯化)

≥12,000 units/mg protein

Product Name: Deoxyribonuclease II from porcine spleen(Purified)

CAS号:9025-64-3
EINECS编号:232-801-8
MDL号:MFCD00130919
介绍:Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.
用途:DNase II has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA). Deoxyribonuclease II from bovine spleen has been used in a study that conducted a partial purification of deoxyribonucleases from eggs and liver of Xenopus laevis. Deoxyribonuclease II from bovine spleen has also been used in a study to investigate nucleic acid and protein synthesis of splenic lymphocytes.
贮存:储存温度 -20°C
生化和生理学机理:The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions. It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.

氧核糖核酸酶Ⅱ 来源于猪脾(纯化)


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