Recombinant High Mobility Group AT Hook Protein 1 (HMGA1) XY92414RPHu01

参考价:¥720
供货周期: 现货
品牌: 信裕生物
规格: 10ug/50ug/200ug/1mg
货号: XY92414RPHu01
CAS号:
上海信裕生物科技有限公司
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Recombinant High Mobility Group AT Hook Protein 1 (HMGA1)

高迁移率族AT Hook蛋白1(HMGA1)重组蛋白

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Glu3~Gln107

Tags: N-terminal His Tag

Subcellular Location: Nucleus, Chromosome

Purity: > 97%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% SKL, 5% Trehalose and Proclin300.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point:

Predicted Molecular Mass: 13.0kDa

Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions.

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. 

Store at 2-8℃ for one month. 

Aliquot and store at -80℃ for 12 months. 

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition. 

[ SEQUENCE ]

[ IDENTIFICATION ]

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