方案摘要
方案下载应用领域 | |
检测样本 | |
检测项目 | |
参考标准 |
The expanding field of monoclonal antibody-based pharmaceuticals has triggered increased interest in analytical characterization of these large proteins and in understanding of their heterogeneity and degradation pathways. As a result, a large number of enzymatic modifications as well as chemical and physical degradations have been reported in monoclonal antibodies in recent years. Most heterogeneity is related to changes in the surface charge of the antibody, either directly, as a change in the number of charged residues, or indirectly as a chemical or physical alteration that changes surface-charge distribution. This review presents an overview of the sources of charge-related heterogeneity in monoclonal antibodies and the methods used for their detection. A detailed section is dedicated to deamidation of asparagine and isomerization of aspartic acid residues, two ubiquitous degradation pathways detected in antibodies and other proteins as well. Finally, kinetic modeling of the accumulation of antibody variants is presented as a tool to determine the expected fraction of molecules that have undergone one or more degradation reactions.
ACE毛细管等电聚焦系统
TSK-GEL G3000SWxl色谱柱的介绍、日常维护及规模放大
TSK-GEL G2000SWxl色谱柱的介绍、日常维护及规模放大
相关产品
TOSOH LenS3多角度光散射检测仪(HPCL&UHPCL系统兼容)
东曹 HLC-8420GPC EcoSEC Elite凝胶渗透色谱仪
高温GPC系统HLC-8321GPC/HT
IC2010 高通量离子色谱仪
guardcolumn DEAE-NPR保护柱
TSKguardcolumn SuperMP-M保护柱
TSKguardcolumn SuperH-L保护柱
TSKguardcolumn SuperH-H保护柱
TSKguardcolumn HHR-L保护柱
TSKguardcolumn HHR-H保护柱
TSKguardcolumn Oligo保护柱
TSKgel NH2系列 保护柱芯
TSKguardcolumn Phenyl-5PW保护柱
TSKgel guardcolumn HXL-L保护柱
TSKgel guardcolumn HXL-H保护柱
关注
拨打电话
留言咨询