钙结合蛋白(CR)检测试剂盒
适用生物 Homo sapiens (Human,人)
钙结合蛋白(CR)检测试剂盒
检测范围 0.156-10ng/mL 灵敏度 0.059ng/mL
样本类型 Serum, plasma, tissue homogenates and other biological fluids
实验时长 4.5h 实验方法 双抗夹心法 钙结合蛋白(CR)检测试剂盒
规格 96T
ELISA Kit for Calretinin (CR)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. hzEA687Hu
Sample type Serum, plasma, tissue homogenates and other biological fluids
Format 96-well strip plate
Assay length 4.5 hours
Detection range 0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL
Sensitivity The minimum detectable dose of this kit is typically less than 0.059ng/mL.
Specificity
This assay has high sensitivity and excellent specificity for detection of Calretinin (CR).
No significant cross-reactivity or interference between Calretinin (CR) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Calretinin (CR) and the recovery rates were calculated by comparing the measured value to the expected amount of Calretinin (CR) in samples.
Matrix Recovery range (%) Average(%)
serum(n=5) 95-103 99
EDTA plasma(n=5) 99-105 102
heparin plasma(n=5) 80-94 90
钙结合蛋白(CR)检测试剂盒Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calretinin (CR) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calretinin (CR) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Calretinin (CR) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-102% 86-102% 92-99% 94-102%
EDTA plasma(n=5) 79-91% 93-101% 81-105% 79-93%
heparin plasma(n=5) 94-102% 79-101% 83-90% 83-101%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B 1×120μL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
钙结合蛋白(CR)检测试剂盒Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50μL Stop Solution. Read at 450nm immediately.
钙结合蛋白(CR)检测试剂盒Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calretinin (CR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calretinin (CR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calretinin (CR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calretinin (CR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Gene/ItemProteinsAntibodiesAssay Kits
Package and Components
Reagent Preparation
Results demonstration
Typical Standard Curve
Certificate
Download
Free use and Guarantees
Free trial
In case it is difficult to pre-evaluate experiment result because of specific test samples or other reasons, free trial can be offered.
If the budget is really limited or other special situation, you can apply the free products.
Guarantees
Unconditionally return policy within 72 hours.
For any complaint, we promise to reply within one work day, resolve the problem within three work days. Implementing initial consultation system in order to provide satisfactory service for all the customers. Customers can unconditionally to request a charge back or refund.
Benchmark price
Please consult local dealer
细胞百科之鳞状上皮细胞
细胞常见问题之细胞培养中遇到细胞空泡化怎么办?
细胞百科之----Vero细胞(非洲绿猴肾细胞)
开学大促 | 惊喜“爆”到!
相关产品
关注
拨打电话
留言咨询