人转录因子AP-1 ELISA kit

上海沪震生物科技有限公司

2015/05/20 16:49

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人转录因子AP-1 ELISA kit

产品类型:

ELISA Kit

产品名称:

人转录因子AP-1 ELISA kit

英文名称:

Human Transcription Factor/Activator Protein 1 (AP-1) ELISA kit

人转录因子AP-1 ELISA kit

别名:

AP-1, AP1, c-Jun, Jun activation domain binding protein|activator protein 1|enhancer-binding protein AP1|v-jun avian sarcoma virus 17 oncogene homolog|v-jun sarcoma virus 17 oncogene homolog

规格:

96T

人转录因子AP-1 ELISA kit

种属:

Human

待测物名称:

jun oncogene

缩写:

JUN

蛋白功能1:

Transcription/Transcription regulation

蛋白功能3:

Transcription

检测范围:

47 pg/ml-3000 pg/ml

灵敏度:

11.75 pg/ml

反应时间:

1-5h

所需样本体积:

50-100ul

检测波长:

450 nm

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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