周期素依赖性激酶2抗体

英文名称    CDK2    

中文名称    周期素依赖性激酶2抗体    

别    名    Cdc2 related protein kinase; cdc2-related protein kinase; Cdk 2; CDK2_HUMAN; Cell devision kinase 2; Cell division kinase 2; Cell division protein kinase 2; Cyclin dependent kinase 2; cyclin dependent kinase 2-alpha; Cyclin-dependent kinase 2; p33 protein kinase; p33(CDK2).    

说 明 书    0.1ml  0.2ml      

研究领域    肿瘤  细胞生物  染色质和核信号  信号转导  细胞周期蛋白  激酶和磷酸酶      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Pig, Cow, Horse, Rabbit,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 周期素依赖性激酶2抗体（石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    33kDa    

细胞定位    细胞核 细胞浆     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human CDK2    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

周期素依赖性激酶2抗体产品介绍    background:

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq, Jul 2008]. 

Function:
Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization.    

周期素依赖性激酶2抗体

hz-K1505 rTaq DNA Polymerase  重组Taq DNA 聚合酶

hz-K1506 rTaq DNA Polymerase  重组Taq DNA 聚合酶

1207-10/50/100  动物RNAout(快速动物总RNA提取试剂)

3080-10/50  植物RNAout（植物RNA提取专用试剂）

3071-10/50  血液RNAout(血液总RNA提取试剂)

3073-10/50  病毒RNAout(病毒RNA提取试剂)

51102-10/100  细菌RNA提取试剂

1206-10/25/50 RNAin  RNAin（非冻型RNA样品保存液）

R1100－20 TRIquick  总RNA提取试剂

R1200 TRIquickLS  液体样品RNA提取试剂

R1300 RNA wait  RNA高效保存液

R1400 RNA plant  植物RNA提取的专用试剂

3130  RNAon（即用型RNA变性上样液）

3140  RNAsharp（RNA电泳带锐化剂）

P1250  蛋白抽提试剂盒-I (实体组织和细胞蛋白抽提)

P1255  蛋白抽提试剂盒-II (液体样品蛋白抽提和回收)

P1200  核-胞浆蛋白制备试剂盒 (Nuclear-Cytosol Extraction Kit)

hz-60  伊红 Y(Eosin Y Sodium)

hz-0001 Peroxidase (POD) Horseradish  辣根过氧化物酶

hz-0061 Trypan Blue  台盼蓝染色液

hz-0062 Trypan Blue  台盼蓝

hz-0063 Toluidine Blue O  甲苯胺蓝

hz-0064 X-gal  

hz-0072 Ponceau S  丽春红S

hz-0028 FITC  异硫氢酸荧光素

hz-0067 p-Nitrophenyl Phosphate Disodium，pNPP  对硝基苯磷酸二钠

hz-0068 MTT  噻唑兰

hz-0069 OPD(o-Phenylenediamine)  邻苯二胺

hz-0083 ThzED  四甲基乙二胺

hz-0084 Activiated Biotin  活化生物素

PH-7 Rabbit IgG Sepharose 4B  兔IgG亲和层析柱

PH-8 Mouse IgG Sepharose 4B  小鼠IgG亲和层析柱

PH-5 Protein G-Sepharose 4B   重组蛋白G亲和层析柱

PH-6 rec Protein A-Sepharose FF  重组蛋白A亲和层析柱