磷酸化信号转导功能磷蛋白DAB2抗体

英文名称    phospho-DAB2 (Ser24)    

中文名称    磷酸化信号转导功能磷蛋白DAB2抗体    

别    名    p-Dab2(Ser24); DAB 2; DAB-2; Differentially expressed protein 2; Disabled (Drosophila) homolog 2 (mitogen responsive phosphoprotein); disabled (Drosophila) homolog 2 (mitogen-responsive phosphoprotein); Disabled homolog 2; Disabled homolog 2 mitogen responsive phosphoprotein (Drosophila); Disabled homolog 2 mitogen responsive phosphoprotein; disabled homolog 2 mitogen-responsive phosphoprotein (Drosophila); DOC 2; DOC2; FLJ26626; Mitogen responsive phosphoprotein; DAB2_HUMAN.    

说 明 书    0.1ml      

产品类型    磷酸化抗体     

研究领域    肿瘤  细胞生物  信号转导  细胞周期蛋白  转录调节因子  激酶和磷酸酶  表观遗传学      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Pig, Cow,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 磷酸化信号转导功能磷蛋白DAB2抗体（石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    85kDa    

细胞定位    细胞浆 细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthesised phosphopeptide derived from human DAB2 around the phosphorylation site of Ser24 [AP(p-S)KK]    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

磷酸化信号转导功能磷蛋白DAB2抗体产品介绍    background:

DAB2 is a component of the CSF1 signal transduction pathway. DAB2 mRNA is expressed in normal ovarian epithelial cells but is down regulated or absent from ovarian carcinoma cell lines. The down regulation of DAB2 may play an important role in ovarian carcinogenesis. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2 like domains, alpha and beta).

Function:
Adapter protein that functions as clathrin-associated sorting protein (CLASP) required for clathrin-mediated endocytosis of selected cargo proteins. Can bind and assemble clathrin, and binds simultaneously to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and cargos containg non-phosphorylated NPXY internalization motifs, such as the LDL receptor, to recruit them to clathrin-coated pits. Can function in clathrin-mediated endocytosis independently of the AP-2 complex. Involved in endocytosis of integrin beta-1; this function seems to redundant with the AP-2 complex and seems to require DAB2 binding to endocytosis accessory EH domain-containing proteins such as EPS15, EPS15L1 and ITSN1. Involved in endocytosis of cystic fibrosis transmembrane conductance regulator/CFTR. Involved in endocytosis of megalin/LRP2 lipoprotein receptor during embryonal development. Required for recycling of the TGF-beta receptor. Involved in CFTR trafficking to the late endosome. Involved in several receptor-mediated signaling pathways. Involved in TGF-beta receptor signaling and facilitates phosphorylation of the signal transducer SMAD2. Mediates TFG-beta-stimulated JNK activation. May inhibit the canoniocal Wnt/beta-catenin signaling pathway by stabilizing the beta-catenin destruction complex through a competing association with axin preventing its dephosphorylation through protein phosphatase 1 (PP1). Sequesters LRP6 towards clathrin-mediated endocytosis, leading to inhibition of Wnt/beta-catenin signaling. May activate non-canonical Wnt signaling. In cell surface growth factor/Ras signaling pathways proposed to inhibit ERK activation by interrupting the binding of GRB2 to SOS1 and to inhibit SRC by preventing its activating phosphorylation at 'Tyr-419'. Proposed to be involved in modulation of androgen receptor (AR) signaling mediated by SRC activation; seems to compete with AR for interaction with SRC. Plays a role in the CSF-1 signal transduction pathway. Plays a role in cellular differentiation. Involved in cell positioning and formation of visceral endoderm (VE) during embryogenesis and proposed to be required in the VE to respond to Nodal signaling coming from the epiblast. Required for the epithelial to mesenchymal transition, a process necessary for proper embryonic development. May be involved in myeloid cell differentiation and can induce macrophage adhesion and spreading. May act as a tumor suppressor. 

Subunit:
Can interact (via PID domain) with LDLR, APP, APLP1 and APLP2, and weakly with INPP5D (via NPXY motifs); the interaction is impaired by tyrosine phosphorylation of the respective NPXY motifs. Can weakly interact (via PID domain) with LRP1 (via NPXY motif); the interaction is enhanced by tyrosine phosphorylation of the NPXY motif. Interacts with LRP2 (via NPXY motif); the interaction is not affected by tyrosine phosphorylation of the NPXY motif. Interacts with clathrin; in vitro can assemble clathrin triskelia into polyhedral coats. Interacts with AP2A2, ITGB1, ITGB3, ITGB5, PIAS2, DAB2IP, NOSTRIN, FCHO1, DVL3, EPS15, ITSN1 and EPS15L1. Interacts with SH3KBP1 (via SH3 domains). Interacts with GRB2; competes with SOS1 for binding to GRB2 and the interaction is enhanced by EGF and NT-3 stimulation. Interacts with MAP3K7; the interaction is induced by TGF-beta stimulation and may mediate TGF-beta stimulated JNK activation. Interacts with AXIN1 and PPP1CA; the interactions are mutually exclusive. Interacts with the globular tail of MYO6. Interacts (via DPF motifs) with FCHO2; the interaction is direct and required for DAB2-mediated LDLR endocytosis. Interacts with LRP6; the interaction involves LRP6 phosphorylation by CK2 and sequesters LRP6 towards clathrin-mediated endocytosis. Associates with the TGF-beta receptor complex (Probable). Interacts with SMAD2 and SMAD3; the interactions are enhanced upon TGF-beta stimulation. Interacts with GRB2; the interaction is enhanced by EGF and NT-3 stimulation. Interacts with SRC; the interaction is enhanced by EGF stimulation. 

Subcellular Location:
Cytoplasmic vesicle; clathrin coated vesicle; clathrin coated vesicle membrane. Cell membrane; coated pit; clathrin coated pit. Note: Colocalizes with large insert containing isoforms of MYO6 at clathrin coated pits/vesicles.

Tissue Specificity:
Expressed in deep invaginations, inclusion cysts and the surface epithelial cells of the ovary. Also expressed in breast epithelial cells, spleen, thymus, prostate, testis, macrophages, fibroblasts, lung epithelial cells, placenta, brain stem, heart and small intestine. Expressed in kidney proximal tubular epithelial cells (at protein level). 

Post-translational modifications:
Phosphorylated. Phosphorylation during mitosis is leading to membrane displacement (By similarity). 

Similarity:
Contains 1 PID domain.     

磷酸化信号转导功能磷蛋白DAB2抗体

hz-0314R-PE-hzy3 Rabbit Anti-chicken IgM/PE-Cy3  PE-Cy3标记的兔抗鸡IgM

hz-0314R-PE-hzY5 Rabbit Anti-chicken IgM/PE-CY5  PE-CY5标记的兔抗鸡IgM

hz-0314R-APhz Rabbit Anti-chicken IgM/APC  APC标记的兔抗鸡IgM

hz-0314R-HRP Rabbit Anti-chicken IgM/HRP  辣根过氧化物酶标记的兔抗鸡IgM

hz-0314R-AP Rabbit Anti-chicken IgM/AP  碱性磷酸酶（AP）标记的兔抗鸡IgM

hz-0314R-AF350 Rabbit Anti-chicken IgM/Alexa Fluor 350  Alexa Fluor 350标记的兔抗鸡IgM

hz-0314R-AF488 Rabbit Anti-chicken IgM/Alexa Fluor 488  Alexa Fluor 488标记的兔抗鸡IgM

hz-0314R-AF555 Rabbit Anti-chicken IgM/Alexa Fluor 555  Alexa Fluor 555标记的兔抗鸡IgM

hz-0314R-AF647 Rabbit Anti-chicken IgM/Alexa Fluor 647  Alexa Fluor 647标记的兔抗鸡IgM

hz-0314R-PE-hzy5.5 Rabbit Anti-chicken IgM/PE-Cy5.5  PE-Cy5.5标记的兔抗鸡IgM

hz-0314R-PE-hzy7 Rabbit Anti-chicken IgM/PE-Cy7  PE-Cy7标记的兔抗鸡IgM

hz-0314R-Bio Rabbit Anti-chicken IgM/Bio  生物素标记的兔抗鸡IgM

hz-0303R-Bio Rabbit Anti-dog IgG/Bio  生物素标记的兔抗狗IgG

hz-0303R-HRP Rabbit Anti-dog IgG/HRP  辣根过氧化物酶标记的兔抗狗IgG

hz-0303R-AP Rabbit Anti-dog IgG/AP  碱性磷酸酶（AP）标记的兔抗狗IgG

hz-0303R-hzy3 Rabbit Anti-dog IgG/Cy3  Cy3标记的兔抗狗IgG

hz-0303R-hzy5 Rabbit Anti-dog IgG/Cy5  Cy5标记的兔抗狗IgG

hz-0303R-hzy5.5 Rabbit Anti-dog IgG/Cy5.5  Cy5.5标记的兔抗狗IgG

hz-0303R-hzy7 Rabbit Anti-dog IgG/Cy7  Cy7标记的兔抗狗IgG

hz-0303R-PE Rabbit Anti-dog IgG/PE  PE标记的兔抗狗IgG

hz-0303R-PE-hzy3 Rabbit Anti-dog IgG/PE-Cy3  PE-Cy3标记的兔抗狗IgG

hz-0303R-PE-hzY5 Rabbit Anti-dog IgG/PE-CY5  PE-CY5标记的兔抗狗IgG

hz-0303R-APhz Rabbit Anti-dog IgG/APC  APC标记的兔抗狗IgG

hz-0303R-AF350 Rabbit Anti-dog IgG/Alexa Fluor 350  Alexa Fluor 350标记的兔抗狗IgG

hz-0303R-AF647 Rabbit Anti-dog IgG/Alexa Fluor 647  Alexa Fluor 647标记的兔抗狗IgG

hz-0303R-PE-hzy5.5 Rabbit Anti-dog IgG/PE-Cy5.5  PE-Cy5.5标记的兔抗狗IgG

hz-0303R-PE-hzy7 Rabbit Anti-dog IgG/PE-Cy7  PE-Cy7标记的兔抗狗IgG

hz-0303R-RBIThz Rabbit Anti-dog IgG/RBITC  罗丹明标记的兔抗狗IgG

hz-0303R-Gold Rabbit Anti-dog IgG/Gold  胶体金标记的兔抗狗IgG

hz-0303R-FIThz Rabbit Anti-dog IgG/FITC  FITC标记的兔抗狗IgG

hz-0303R-AF488 Rabbit Anti-dog IgG/Alexa Fluor 488  Alexa Fluor 488标记的兔抗狗IgG

hz-0303R-AF555 Rabbit Anti-dog IgG/Alexa Fluor 555  Alexa Fluor 555标记的兔抗狗IgG