供货周期: | 现货 |
品牌: | R&D |
规格: | g/mg |
货号: | hz-1639R |
CAS号: |
英文名称 phospho-FAK(Tyr577)
中文名称 磷酸化粘着斑激酶抗体
别 名 FAK (phospho Y577); p-FAK (phospho Y577); FADK 1; FADK; FAK 1; FAK related non kinase polypeptide; FAK1; Focal adhesion kinase 1; FRNK; pp125FAK; Protein tyrosine kinase 2; Protein Tyrosine Kinase Cytoplasmic; PTK 2; FAK1_HUMAN; Focal adhesion kinase-related nonkinase; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK.
说 明 书 0.1ml
产品类型 磷酸化抗体
研究领域 肿瘤 细胞生物 神经生物学 信号转导 激酶和磷酸酶
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 磷酸化粘着斑激酶抗体(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 116kDa
细胞定位 细胞核 细胞浆 细胞膜
性 状 Lyophilized or Liquid
浓 度 1mg/1ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human FAK around the phosphorylation site of Tyr577
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
磷酸化粘着斑激酶抗体产品介绍 background:
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity. [SUBCELLULAR LOCATION] Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Constituent of focal adhesions.
Function:
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Subunit:
Interacts (via first Pro-rich region) with CAS family members (via SH3 domain), including BCAR1, BCAR3, CASS4 and NEDD9. Interacts with GIT1. Interacts with SORBS1. Interacts with RGNEF. Interacts with SHB. Interacts with PXN and TLN1. Interacts with STAT1. Interacts with DCC. Interacts with WASL. Interacts with ARHGEF7. Interacts with GRB2 and GRB7 (By similarity). Component of a complex that contains at least FER, CTTN and PTK2/FAK1. Interacts with BMX. Interacts with TGFB1I1. Interacts with STEAP4. Interacts with ZFYVE21. Interacts with ESR1. Interacts with PIK3R1 or PIK3R2. Interacts with SRC, FGR, FLT4 and RET. Interacts with EPHA2 in resting cells; activation of EPHA2 recruits PTPN11, leading to dephosphorylation of PTK2/FAK1 and dissociation of the complex. Interacts with EPHA1 (kinase activity-dependent). Interacts with CD4; this interaction requires the presence of HIV-1 gp120. Interacts with PIAS1. Interacts with ARHGAP26 and SHC1. Interacts with RB1CC1; this inhibits PTK2/FAK1 activity and activation of downstream signaling pathways. Interacts with P53/TP53 and MDM2. Interacts with LPXN (via LD motif 3).
Subcellular Location:
Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Nucleus. Note=Constituent of focal adhesions. Detected at microtubules.
Tissue Specificity:
Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous.
Post-translational modifications:
Phosphorylated on tyrosine residues upon activation, e.g. upon integrin signaling. Tyr-397 is the major autophosphorylation site, but other kinases can also phosphorylate this residue. Phosphorylation at Tyr-397 promotes interaction with SRC and SRC family members, leading to phosphorylation at Tyr-576, Tyr-577 and at additional tyrosine residues. FGR promotes phosphorylation at Tyr-397 and Tyr-576. FER promotes phosphorylation at Tyr-577, Tyr-861 and Tyr-925, even when cells are not adherent. Tyr-397, Tyr-576 and Ser-722 are phosphorylated only when cells are adherent. Phosphorylation at Tyr-397 is important for interaction with BMX, PIK3R1 and SHC1. Phosphorylation at Tyr-925 is important for interaction with GRB2. Dephosphorylated by PTPN11; PTPN11 is recruited to PTK2 via EPHA2 (tyrosine phosphorylated). Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly; this dephosphorylation could be catalyzed by PTPN11 and regulated by ZFYVE21.
Sumoylated; this enhances autophosphorylation.
DISEASE:
Note=Aberrant PTK2/FAK1 expression may play a role in cancer cell proliferation, migration and invasion, in tumor formation and metastasis. PTK2/FAK1 overexpression is seen in many types of cancer.
Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family.
FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
磷酸化粘着斑激酶抗体
hz-1090 BAFF-R(Human B cell activation factorr from the tumor necrosis factor family receptor) ELISA Kit 人B细胞活化因子受体
hz-1090 HSF1(Mouse Heat Shock Factor 1) ELISA Kit 小鼠热休克因子1
hz-1091 VEGFR-3/Flt-4(Human Vascuoar endothelial cell growth factor receptor 2) ELISA kit 人血管内皮细胞生长因子受体3
hz-1091 IAA ELISA Kit 大鼠胰岛素自身抗体
hz-1091 PF3(Mouse platelet factor 3) ELISA Kit 小鼠血小板因子3
hz-1092 gp210 ELISA Kit 大鼠抗核膜糖蛋白210抗体
hz-1092 IL-2R(Mouse Interleukin-2 receptor) ELISA kit 小鼠白介素2受体
hz-1092 VEGFR-1/Flt1(Human Vascuoar endothelial cell growth factor receptor 1) ELISA kit 人血管内皮细胞生长因子受体1
hz-1093 sTLR6 ELISA Kit 大鼠可溶性Toll样受体6
hz-1093 VEGF-D(Human Vascular Endothelial cell Growth Factor D) ELISA KIT 人血管内皮细胞生长因子D
hz-1093 ADM(Mouse adrenomedullin) ELISA Kit 小鼠肾上腺髓质素
hz-1094 VEGF-C(Human Vascular Endothelial cell Growth Factor C) ELISA KIT 人血管内皮细胞生长因子C
hz-1094 sTLR2 ELISA Kit 大鼠可溶性Toll样受体2
hz-1094 sLR(Mouse Leptin Soluble Receptor) ELISA Kit 小鼠可溶性瘦素受体
hz-1095 VEGF-B(Human Vascular Endothelial cell Growth Factor B) ELISA KIT 人血管内皮细胞生长因子B
hz-1095 PGF2 Alpha ELISA Kit 大鼠前列腺素F2α
hz-1095 Beta-EPR(Mouse Beta-Endorphin receptor) ELISA Kit 小鼠β内啡肽受体
hz-1096 VEGF(Human Vascular Endothelial cell Growth Factor) ELISA KIT 人血管内皮细胞生长因子
hz-1096 LTE4 ELISA Kit 大鼠白三烯E4
hz-1096 HL(Mouse hepatic lipase) ELISA Kit 小鼠肝脂酶
hz-1097 UK ELISA Kit 大鼠尿激酶
hz-1097 VCAM-1/CD106(Human Vascuolar cell adhesion molecule 1) ELISA kit 人血管内皮细胞粘附分子1
hz-1097 HBcAb(Mouse hepatitis B virus core antibody) ELISA Kit 小鼠乙型肝炎核心抗体
hz-1098 HBeAb(Mouse hepatitis B virus e antibody) ELISA Kit 小鼠乙型肝炎e抗体
hz-1098 sTRAIL(Human soluble tumor necrosis factor-related apoptosis inducing ligand) ELISA KIT 人可溶性肿瘤坏死因子相关凋亡诱导配体
hz-1098 F XII ELISA Kit 大鼠凝血因子Ⅻ
hz-1099 F X III ELISA Kit 大鼠凝血因子ⅩⅢ
hz-1099 TRAIL-R4(Human tumor necrosis factor-related apoptosis-inducing ligand 4) ELISA Kit 人肿瘤坏死因子相关凋亡诱导配体4
hz-1099 HBeAg(Mouse hepatitis B virus e antigen) ELISA Kit 小鼠乙型肝炎e抗原
hz-1100 TRAIL-R3(Human tumor necrosis factor-related apoptosis-inducing ligand 3) ELISA Kit 人肿瘤坏死因子相关凋亡诱导配体3
hz-1100 F III ELISA Kit 大鼠凝血因子Ⅲ
hz-1100 HBsAb(Mouse hepatitis B virus surface antibody) ELISA Kit 小鼠乙型肝炎表面抗体
相关产品
关注
拨打电话
留言咨询