| 供货周期： | 现货 |
| 品牌： | 沃特世 |
| 货号： | 186003582 |
BEH130 C18和 BEH300 C18，广泛通用，耐受性强！
? 基于亚乙基桥杂化颗粒BEH，pH 1-12，耐受性强，高盐或高温条件下柱寿命更长?
与硅胶C18柱相比，活性硅醇基残留显著减少，能够提供更为尖锐、对称的峰形?
通用性强，广泛适用于各种肽的分析与分离?
提供两种孔径，130?和300?，以适应个别项目的特定需求?
涵盖不同规格色谱柱，包括：纳米级毛细管柱、UPLC柱、HPLC分析柱、OBD制备柱，满足从纳米级分析到实验室级别纯化的各阶段所需。
?
使用细胞色素C胰蛋白酶解物标准品、在0.1%TFA流动相条件下，对色谱柱填料批次进行质量控制，确保对验证方法提供稳定一致的柱间重现性。
BEH130 C18与BEH300
C18的柱耐受性考察(低pH条件下加速)
色谱柱会因为物理原因和化学原因而失效。在低pH条件下，柱寿命短的主要原因是键合相的水解，这会导致肽保留的显著改变。BEH技术色谱柱包含了沃特世专有的键合与封端技术，能够产生稳定的键合相。在低pH条件下稳定性测试中，BEH
C18柱显示出极少的保留损失。
经过严格测试以确保填料批次的可重现性
每一批ACQUITY UPLC和XBridge BEH 130和BEH300
C18填料，都经过严格测试以确保持续一致的色谱性能。每根色谱柱的产品分析证书，都报告了该批次色谱填料的物理、化学和色谱测试结果，包括使用牛细胞色素C的胰蛋白酶解物作为混标检测的分离结果。
为肽的纯化简化色谱柱选择
可以使用单一C18柱，分离广泛范围下的各种肽。在UPLC柱或HPLC分析柱上所得的条件与分离，可以很方便的放大到相应的OBD制备柱上用于制备纯化，不同粒径的填料化学选择性完全一致。OBD技术(Optimum
Bed Density，最优柱床密度)，沃特世通过这项专利技术，以确保获得最稳定的填充柱床，从而延长制备柱的柱寿命(物理稳定性)。
肽分离技术制备柱选择指南
Prep OBD柱，5μm和10μm 130A 和 300A
| 内径 (mm) | 长度 (mm) | 单个肽μmol量 | 单个肽重量 (mg) | 典型流速 (mL/min) |
| 10 | 50 | 0.25–5 | 0.5–10 | 4.5–9 |
| 10 | 100 | 0.25–5 | 0.5–10 | 4.5–9 |
| 10 | 150 | 0.25–5 | 0.5–10 | 4.5–9 |
| 10 | 250 | 0.25–5 | 0.5–10 | 4.5–9 |
| 19 | 50 | 1–18 | 2.0–36 | 16–32 |
| 19 | 100 | 1–18 | 2.0–36 | 16–32 |
| 19 | 150 | 1–18 | 2.0–36 | 16–32 |
| 19 | 250 | 1–18 | 2.0–36 | 16–32 |
Prep OBD 柱，10 μm 130A 和 300A
| 内径 (mm) | 长度 (mm) | 单个肽μmol量 | 单个肽重量 (mg) | 典型流速 (mL/min) |
| 30 | 50 | 2.5–25 | 5–100 | 40–80 |
| 30 | 100 | 2.5–25 | 5–100 | 40–80 |
| 30 | 150 | 2.5–25 | 5–100 | 40–80 |
| 30 | 250 | 2.5–25 | 5–100 | 40–80 |
XBridge Peptide BEH C18, 130? and 300? HPLC 和UHPLC制备柱
| 产品名称 | 粒径 | 内径 | 径长 | 规格 | 部件编号 |
| XBridge Peptide BEH C18, 130A | 5 μm | 10 mm | 50 mm | Column | 186003582 |
| XBridge Peptide BEH C18, 130A | 5 μm | 10 mm | 100 mm | Column | 186003583 |
| XBridge Peptide BEH C18, 130A | 5 μm | 10 mm | 150 mm | Column | 186003584 |
| XBridge Peptide BEH C18, 130A | 5 μm | 10 mm | 250 mm | Column | 186003585 |
| XBridge Peptide BEH C18, 130A | 5 μm | 19 mm | 50 mm | OBD? Column | 186003586 |
| XBridge Peptide BEH C18, 130A | 5 μm | 19 mm | 100 mm | OBD Column | 186003587 |
| XBridge Peptide BEH C 18, 130A | 5 μm | 19 mm | 150 mm | OBD Column | 186003945 |
| XBridge Peptide BEH C 18, 130A | 10 μm | 4.6 mm | 50 mm | Column | 186003648 |
| XBridge Peptide BEH C18, 130A | 10 μm | 4.6 mm | 100 mm | Column | 186003649 |
| XBridge Peptide BEH C18, 130A | 10 μm | 4.6 mm | 150 mm | Column | 186003650 |
| XBridge Peptide BEH C18, 130A | 10 μm | 4.6 mm | 250 mm | Column | 186003651 |
| XBridge Peptide BEH C18, 130A | 10 μm | 10 mm | 50 mm | Column | 186003652 |
| XBridge Peptide BEH C18, 130A | 10 μm | 10 mm | 100 mm | Column | 186003653 |
| XBridge Peptide BEH C18, 130A | 10 μm | 10 mm | 150 mm | Column | 186003654 |
| XBridge Peptide BEH C18, 130A | 10 μm | 10 mm | 250 mm | Column | 186003655 |
| XBridge Peptide BEH C18, 130A | 10 μm | 19 mm | 50 mm | OBD Column | 186003656 |
| XBridge Peptide BEH C18, 130A | 10 μm | 19 mm | 150 mm | OBD Column | 186003657 |
| XBridge Peptide BEH C18, 130A | 10 μm | 19 mm | 250 mm | OBD Column | 186003658 |
| XBridge Peptide BEH C18, 130A | 10 μm | 30 mm | 50 mm | OBD Column | 186003659 |
| XBridge Peptide BEH C18, 130A | 10 μm | 30 mm | 100 mm | OBD Column | 186003660 |
| XBridge Peptide BEH C18, 130A | 10 μm | 30 mm | 150 mm | OBD Column | 186003661 |
| XBridge Peptide BEH C18, 130A | 10 μm | 30 mm | 250 mm | OBD Column | 186003662 |
| XBridge Peptide BEH C18, 300A | 5 μm | 10 mm | 50 mm | Column | 186003626 |
| XBridge Peptide BEH C18, 300A | 5 μm | 10 mm | 100 mm | Column | 186003627 |
| XBridge Peptide BEH C18, 300A | 5 μm | 10 mm | 150 mm | Column | 186003628 |
| XBridge Peptide BEH C18, 300A | 5 μm | 10 mm | 250 mm | Column | 186003629 |
| XBridge Peptide BEH C18, 300A | 5 μm | 19 mm | 50 mm | OBD Column | 186003630 |
| XBridge Peptide BEH C18, 300A | 5 μm | 19 mm | 100 mm | OBD Column | 186003631 |
| XBridge Peptide BEH C18, 300A | 5 μm | 19 mm | 150 mm | OBD Column | 186003946 |
| XBridge Peptide BEH C18, 300A | 10 μm | 4.6 mm | 50 mm | Column | 186003663 |
| XBridge Peptide BEH C18, 300A | 10 μm | 4.6 mm | 100 mm | Column | 186003664 |
| XBridge Peptide BEH C18, 300A | 10 μm | 4.6 mm | 150 mm | Column | 186003665 |
| XBridge Peptide BEH C18, 300A | 10 μm | 4.6 mm | 250 mm | Column | 186003666 |
| XBridge Peptide BEH C 18, 300A | 10 μm | 10 mm | 50 mm | Column | 186003667 |
| XBridge Peptide BEH C18, 300A | 10 μm | 10 mm | 100 mm | Column | 186003668 |
| XBridge Peptide BEH C18, 300A | 10 μm | 10 mm | 150 mm | Column | 186003669 |
| XBridge Peptide BEH C18, 300A | 10 μm | 10 mm | 250 mm | Column | 186003670 |
| XBridge Peptide BEH C18, 300A | 10 μm | 19 mm | 50 mm | OBD Column | 186003671 |
| XBridge Peptide BEH C18, 300A | 10 μm | 19 mm | 150 mm | OBD Column | 186003672 |
| XBridge Peptide BEH C18, 300A | 10 μm | 19 mm | 250 mm | OBD Column | 186003673 |
| XBridge Peptide BEH C18, 300A | 10 μm | 30 mm | 50 mm | OBD Column | 186003674 |
| XBridge Peptide BEH C18, 300A | 10 μm | 30 mm | 100 mm | OBD Column | 186003675 |
| XBridge Peptide BEH C18, 300A | 10 μm | 30 mm | 150 mm | OBD Column | 186003676 |
| XBridge Peptide BEH C18, 300A | 10 μm | 30 mm | 250 mm | OBD Column | 186003677 |
以下为制备柱保护柱芯，需配合相应制备保护柱卡套使用
| 产品名称 | 粒径 | 柱内径 | 柱长 | 规格 | 部件编号 |
| XBridge Peptide BEH130 C18 | 5 μm | 10 mm | 10 mm | 保护柱芯 | 186004469 |
| XBridge Peptide BEH130 C 18 | 5 μm | 19 mm | 10 mm | 保护柱芯 | 186004468 |
| XBridge Peptide BEH130 C18 | 10 μm | 10 mm | 10 mm | 保护柱芯 | 186004465 |
| XBridge Peptide BEH130 C18 | 10 μm | 19 mm | 10 mm | 保护柱芯 | 186004464 |
| XBridge Peptide BEH300 C18 | 5 μm | 10 mm | 10 mm | 保护柱芯 | 186004471 |
| XBridge Peptide BEH300 C 18 | 5 μm | 19 mm | 10 mm | 保护柱芯 | 186004470 |
| XBridge Peptide BEH300 C18 | 10 μm | 10 mm | 10 mm | 保护柱芯 | 186004467 |
| XBridge Peptide BEH300 C18 | 10 μm | 19 mm | 10 mm | 保护柱芯 | 186004466 |
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