GlycanPac AXH-1 多糖分析柱 082470

GlycanPac AXH-1 多糖分析柱

Thermo Scientific™ GlycanPac™ AXH-1 LC 色谱柱适用于生物分子中存在的不带电荷和带电荷多糖分子的定性分析、定量分析以及结构表征。 此款高性能、以硅胶为填料的高效液相色谱 (HPLC) 柱可以同时通过电荷、尺寸和极性对多糖分子进行分离。 可以通过 LC-荧光和 LC-质谱 (MS) 方法对标记或天然的生物学上重要的多糖分子进行高分离度和高通量分析，并具备独特的选择性。

描述

GlycanPac AXH-1 LC 色谱柱利用创新的混合模式表面化学特性，以及弱阴离子交换 (WAX) 和亲水作用色谱（HILIC）保留机理，以实现最佳选择性和分离度。 WAX 功能可以为带有负电荷的多糖提供保留性和选择性，同时 HILIC 模式可以根据其极性和分子尺寸，分离带相同电荷的多糖。 因此，GlycanPac AXH-1 LC 色谱柱可以提供无与伦比的多糖分离能力。

基于电荷、分子尺寸和极性的独特多糖选择性

• 对天然和标记的多糖均具有出色的分离度

• 对于高分离度多糖特性表征和基于电荷的简易多糖定量分析均具有重要作用

• 兼容荧光和 MS 检测方法

• 高色谱效率和出色的色谱柱稳定性

GlycanPac AXH-1 LC 色谱柱以高纯度和球型硅胶基质为填料，具有 1.9 和 3μm 两种规格，分别适合 UHPLC 和标准 HPLC 应用。 GlycanPac AXH-1 LC 色谱柱可以满足您应用中的不同分离度、通量和仪器要求的需求。

GlycanPac AXH-1 多糖分析柱

订货信息：

GlycanPac AXH-1 Columns for the High-Resolution Separation and Analysis of Glycans

The Thermo Scientific^™ GlycanPac^™ AXH-1 column is a high-performance, silica-based HPLC column for simultaneous separation of glycans by charge, size, and polarity. It is designed for high-resolution and high-throughput analysis with unique selectivity for biologically important glycans, either labeled or native, by LC-fluorescence and LC-mass spectrometry (MS) methods.

The GlycanPac AXH-1 column can be used for qualitative, quantitative, and structural characterization of uncharged and charged glycans present in biological molecules (e.g., proteins).

Features:

• Unique glycan selectivity based on charge, size, and polarity

• Excellent resolution for both native and labeled glycans

• Useful for both high-resolution glycan profile characterization and easy quantification of glycans based on charge

• Compatible with fluorescence and MS detection methods

• High chromatographic efficiency and excellent column stability

The GlycanPac AXH-1 column, based on innovative mixed-mode surface chemistry, combines both weak anion-exchange (WAX) and hydrophilic interaction liquid chromatography (HILIC) retention mechanisms for optimal selectivity and resolution power. The WAX functionality provides retention and selectivity for negatively charged glycans, while the HILIC mode facilitates the separation of glycans of the same charge according to their polarity and size. As a result, the GlycanPac AXH-1 column provides unparalleled capabilities for glycan separations. Additionally, this column offers charge-based separations for various glycans without discrimination of size and polarity, making it a suitable tool for accurate quantification of glycans which cannot be achieved with other HPLC columns. The GlycanPac AXH-1 column is designed and tested for LC-fluorescence detection and LC-MS applications using volatile aqueous buffers (e.g., ammonium acetate or ammonium formate) and acetonitrile.

GlycanPac AXH-1 columns are based on high-purity and spherical silica substrates in both 1.9 and 3 μm sizes for UHPLC and standard HPLC applications, respectively. GlycanPac AXH-1 columns are available in various dimensions for applications with different resolutions, throughput, and instrument requirements.

The GlycanPac AXH-1 column can be used for qualitative, quantitative, and structural characterization of uncharged and charged glycans present in biological molecules. The glycan elution profile consists of a series of peaks grouped into several "clusters" in which the neutral glycans elute first, followed by mono-sialylated, di-sialylated, tri-sialylaled species.Analytes in each cluster, retained by anion exchange interactions, represents the glycans of the same charge. Within each cluster the glycans having the same charge are further separated according to their sizes and polarity by HILIC interaction.

Similar separation of N-glycans using a 3μm packing material with Fluorescence detection.

Separation of N-glycans using the GlycanPac AXH-1, 1.9μm packing with LC/MS detection.

Accurate and easy quantitative analysis of relative amounts of glycans of each charge state is possible by using a modified gradient.

Separation of human IgG using the GlycanPac AXH-1, 1.9μm column.

DataSheet
	GlycanPac AXH-1 Column

Brochures
	Thermo Scientific BioLC Columns

Manuals
	Thermo Scientific GlycanPac AXH-1 Columns Product Manual
	GLYCANPAC AXH-1 COLUMN Quick Start

Posters
	Novel Glycan Column Technology for the LC-MS Analysis of Labeled and Native N-Glycans Released from Proteins and Antibodies