DNAPac PA200 寡核苷酸柱

DNAPac PA200 寡核苷酸柱

使用 Thermo Scientific DNAPac PA200 寡核苷酸柱，可实现对合成型寡核苷酸进行高分离度分析和纯化所需的强阴离子交换。

描述

• 可对寡核苷酸进行 n、n-1 分离

• 解析次级结构的寡核苷酸

• 分析硫代磷酸酯纯度

• 通过调节洗脱液 pH、盐和溶剂来进行选择性控制

• 可解析具有不同于正常 SSRNA 的异常链 (2' – 5') 的 RNA

DNAPac PA200 寡核苷酸柱

订货信息：

Ultrahigh-Resolution Separations of Oligonucleotides

The Thermo Scientific^™ DNAPac^™ PA200 and PA200 RS are strong anion-exchange columns developed to provide unsurpassed high-resolution analysis and purification of synthetic oligonucleotides. Both supports are designed to resolve full length from n–1, n+1, and other failure sequences not possible with other columns. Retention times and selectivity can be controlled by choice of salt, pH, and solvent. Therefore, the separation can be tailored to the requirements of many different oligonucleotide analysis challenges. 

• Achieve n, n-1 resolution for oligonucleotides.

• Resolve oligonuleotide linkage and stereoisomers.

• Assay phosphorothioate purity.

• Attain selectivity control with eluent pH, salt, and solvent.

• Resolve RNA with aberrant (2’–5’) links from normal SSRNA.

The DNAPac PA200 is packed with a pellicular anion-exchange resin composed of an 8 μm diameter nonporous polymeric substrate to which quaternary amine-functionalized Thermo Scientific^™ Dionex^™ MicroBeads^™ are bound. The rapid mass transport characteristics of this resin result in high-resolution oligonucleotide separations. 

The DNAPac PA200 RS is smaller, with 4 μm diameter resin for bio-UHPLC separations. These rapid separation (RS) columns are designed for use with the Thermo Scientific^™ Dionex^™ UltiMate^™ 3000 BioRS system, with an operational backpressure limit of 10,000 psi. Columns packed with smaller particles improve resolution and offer better performance.

The DNAPac PA200 and PA 200 RS can be operated under denaturing conditions, such as high temperature (up to 85 °C), high-pH eluents (up to pH 12.5), or by the inclusion of chaotropic agents (such as Urea).

These columns offer the highest-quality phase stability over a broad pH range, deliver exceptional resolution, and support high-throughput separations. DNAPac PA200 and PA 200 RS columns support resolution of normal-length oligonucleotides (8- to 30- mer), extended-length oligonucleotides (30- to 70-mer), linear double-stranded DNA, and supercoiled versus nicked/linear DNA.

Analytical separations on the DNAPac PA200, 8μm, 4 mm diameter column can be scaled directly to larger diameter columns so preparative methods can be conveniently developed using small samples. Scaling the flow rate and sample size up for the 9 × 250 mm and 22 × 250 mm column yields essentially identical chromatography.

Resolution of oligonucleotides using DNAPac PA200 column (8μm).

Separation of oligonucleotides by length using DNAPac PA200 column (8μm).

Influence of pH on Oligonucleotide Retention: NaCl Eluent ±20% CH₃CN.

Improved Stability to Alkaline Conditions: Comparison of Capacity upon Exposure to pH 12.4 at Elevated Temperature.

Improved oligonucleotide sensitivity using the 4μm DNAPac PA200 RS column.

Fast separation of 46 oligonucleotides using the 4μm, DNAPac PA200 RS column.

Comparison of 4μm, DNAPac PA200 RS column throughput.

Separation of three identical sequence oligonucleotides harboring zero, one, or two 2',5'-linkages using the 4μm, DNAPac PA200 RS columns of different lengths. 

Product Data Sheets
	DNAPac PA200 Column Data Sheet

Guides
	DNAPac PA200 Analytical Column Manual
	DNAPac PA200 RS 4μm Columns Quick Start

Applications
	AN 21093: Ultra-High-Resolution Separation of Oligonucleotides on Pellicular Anion-Exchange UHPLC Columns
	AN 20996: High-Resolution Separation of Oligonucleotides on a Pellicular Anion-Exchange Column
	AN 1021: Pellicular Anion-Exchange Oligonucleotide Chromatography Coupled with High-Resolution Accurate Mass (HRAM) Mass Spectrometry
	AN 162: Determination of Nucleotides by Ion Chromatography with UV Absorbance Detection

Articles
	High-Resolution Nucleic Acid Separations by High-Performance Liquid Chromatography

Posters
	Performance Improvements for High Resolution Anion-Exchange Oligonucleotide Separations Using Small Particle Substrates
	Chromatographic Assessment of Oligonucleotide Purity
	Identification of RNA Linkage Isomers by Anion-Exchange Purification with ESI-MS of Automatically-Desalted Phosphodiesterase-II Digests
	High-Resolution RNA and DNA Analyses by Anion-Exchange Liquid Chromatography-Mass Spectrometry
	Implementing Novel Technologies in Diagnostic and Therapeutic Oligonucleotide Development
	On-Line Preparation of Anion-Exchange Separated Oligonucleotides for Mass Spectrometry
	High-speed, High-resolution Oligonucleotide Separations Using Small Particle Anion-Exchangers