供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 96T/48T |
货号: | KL-TIMP4-Hu |
CAS号: |
人组织金属蛋白酶抑制因子4检测试剂盒都是一个试剂盒对应检测一个指标。不存在可以用一个试剂盒同时检测出多个指标。进口原装的有96T的,进口分装的有48T 和 96T的 ,这个得根据老师自己的需要来选择试剂盒的大小和规格,一般96T的可以测85个样本,48T的可以做37次实验,ELISA试剂盒还有11个孔是做标准曲线分析实验数据用的。试剂盒选购好了之后就可以开始测了,ELISA实验必要的一种仪器-----酶标仪!
人组织金属蛋白酶抑制因子4检测试剂盒问:
1 请问为什么有的试剂盒要求反应过夜,而有的则只需要1h?反应时间不同是由什么决定的呢?
2 稀释的抗体如何保持其稳定性?
3 显色体系(加H2O2后)的稳定性如何保持?
4 如何克服批间差异较大的缺陷?
人组织金属蛋白酶抑制因子4检测试剂盒答:
确定的反应工艺有关,一般37度1小时足够了。
稀释道抗体的话,一般都会在稀释液中增加一些无关蛋白,使稀释液有一定的蛋白浓度,也可以添加一些蛋白稳定剂,但关键还是看抗体本身的稳定性怎样。
一般目前国内的显色系统都是将TMB(OPD)和过氧化氢放开保存的。
批间差异大的问题,第一就是要原料质量过关,原料的批和批之间差异不能太大,还有就是用于生产的工艺要简单,人组织金属蛋白酶抑制因子4检测试剂盒重复性好,这样可以尽量减少批间差异。
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