腺苷受体蛋白抗体

Quantity size:

0.2ml

Concentration:

1mg/ml 腺苷受体蛋白抗体  Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Background:

Escherichia coli contains more than 250 known promoters that must be activated prior to transcription. cAMP receptor protein (crp), also known as cAMP regulatory protein and catabolite gene activator,腺苷受体蛋白抗体  is a 210 amino acid E. coli protein that complexes with cyclic AMP and binds to specific DNA sites near the promoter, resulting in homodimeric transcription activation of more than 100 E. coli promoters. Acting as a negative regulator against its own synthesis, crp regulates transcription of several catabolite-sensitive operons. Crp activates transcription by making multiple腺苷受体蛋白抗体  interactions with RNA polymerase. Containing one cyclic nucleotide binding domain and one HTH crp-type DNA binding domain, crp binds DNA as a dimer to induce a severe bend in the DNA. This formation is a regulatory mechanism of crp gene expression that plays a major role in determining the molecular mechanism in gene transcription.

Also known as:

腺苷受体蛋白抗体 CRP_ECOLI; cAMP-activated global transcriptional regulator CRP; Catabolite activator protein; CAP; Catabolite gene activator; cAMP receptor protein; CRP; cAMP regulatory protein.

Specificity:

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Rabbit Polyclonal IgG, affinity purified by Protein A.

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Reacts with: E. coli origin.

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Immunogen: KLH conjugated synthetic peptide derived from human cAMP-receptor protein.

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腺苷受体蛋白抗体 Predicted Molecular Weight: 24kDa.

Storage:

Shipped at 4℃, Store at -20℃ (Avoid repeated freeze/thaw cycles).

Application:

WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500   
Not yet tested in other applications. 
Optimal working dilutions must be determined by the end user.

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