氨酰tRNA合成酶复合多功能相互作用蛋白1(AIMP1)重组蛋白

氨酰tRNA合成酶复合多功能相互作用蛋白1(AIMP1)重组蛋白    

物种Homo sapiens (Human，人)

来源原核表达

宿主E.coli

内毒素水平<1.0EU/μg(LAL法测定)

亚细胞定位n/a

预测分子量19.6kDa

实际分子量-(差异分析请参阅说明书)

片段与标签Lys148~Lys312 (Accession # Q12904) with N-terminal His Tag

缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）

性状冻干粉

氨酰tRNA合成酶复合多功能相互作用蛋白1(AIMP1)重组蛋白 纯度> 95%

等电点-

应用SDS-PAGE; WB; ELISA; IP.

Aminoacyl Aminoacyl Aminoacyl Aminoacyl tRNA Synthetase Synthetase Synthetase Synthetase Complex Complex Complex Complex Interacting Interacting Interacting Interacting Multifunctional Multifunctional Multifunctional Multifunctional Protein Protein Protein Protein 1(AIMP1) 1(AIMP1) 1(AIMP1) 1(AIMP1)

Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)

Instruction Instruction Instruction Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

5th Edition (Revised in January, 2013)

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Aminoacyl tRNA Synthetase

Complex Interacting Multifunctional Protein 1

Synonyms: Synonyms: Synonyms: Synonyms: AIMP1, EMAP2, SCYE1

Species: Species: Species: Species: Human

Size: 100μg

氨酰tRNA合成酶复合多功能相互作用蛋白1(AIMP1)重组蛋白 Source: Source: Source: Source: Escherichia coli-derived

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Nucleus. Cytoplasm, cytosol.

Cytoplasmic vesicle, secretory vesicle. Secreted.

Endoplasmic reticulum. Golgi apparatus.

[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]

Residues: Residues: Residues: Residues: Lys148~Lys312 (Accession # Q12904),

with N-terminal His-Tag. Grade & Purity: Purity: Purity: Purity: >95%, 22kDa as determined by

SDS-PAGE reducing conditions.

Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS, pH

7.4, containing 5% sucrose, 0.01% sarcosyl. Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by

the LAL method).

Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 19.6kDa

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 6.8

Human AIMP1

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

氨酰tRNA合成酶复合多功能相互作用蛋白1(AIMP1)重组蛋白 [ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF- KPI DVSRLDLRIG CIITARKHPD ADSLYVEEVD VGEIAPRTVV

SGLVNHVPLE QMQNRMVILL CNLKPAKMRG VLSQAMVMCA SSPEKIEILA PPNGSVPGDR

ITFDAFPGEP DKELNPKKKI WEQIQPDLHT NDECVATYKG VPFEVKGKGV CRAQTMSNSG

IK