信号素5B(SEMA5B)重组蛋白

参考价:面议
供货周期: 现货
品牌: 康朗生物
规格: 50ug/100ug/250ug
货号: KL926Hu01
CAS号:
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信号素5B(SEMA5B)重组蛋白

来源原核表达

宿主E.coli

内毒素水平<1.0EU/μg(LAL法测定)

亚细胞定位细胞膜

预测分子量14.9kDa

实际分子量14kDa(差异分析请参阅说明书)

片段与标签Leu36~Glu161 with N-terminal His Tag

缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)

性状冻干粉

纯度> 95%

等电点6.0

信号素5B(SEMA5B)重组蛋白应用SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling

RPL926Hu01 100μg

Recombinant Semaphorin 5B (SEMA5B)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

11th Edition (Revised in May, 2016)

信号素5B(SEMA5B)重组蛋白[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Leu36~Glu161

Tags: N-terminal His-Tag

Tissue Specificity: Brain, Ovary, Lung. Subcellular Location: Membrane; Single-pass type III membrane protein. Purity: >95%

Traits: Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01%

sarcosyl and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification;

Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.0

Predicted Molecular Mass: 14.9kDa

Accurate Molecular Mass: 14kDa as determined by SDS-PAGE reducing conditions. [ USAGE ]

Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

信号素5B(SEMA5B)重组蛋白[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate

was determined by accelerated thermal degradation test, that is, incubate the

protein at 37

oC for 48h, and no obvious degradation and precipitation were

observed.The loss rate is less than 5% within the expiration date under

appropriate storage condition. [ SEQUENCE ]

[ IDENTIFICATION ]

Figure 1. SDS-PAGE


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