结肠癌转移关联基因1(MACC1)重组蛋白

结肠癌转移关联基因1(MACC1)重组蛋白

来源原核表达

宿主E.coli

内毒素水平<1.0EU/μg(LAL法测定)

亚细胞定位n/a

预测分子量57.2kDa

实际分子量-(差异分析请参阅说明书)

片段与标签Gln522-Met784 (Accession # Q6ZN28) with N-terminal His Tag

缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）

性状冻干粉

纯度> 95%

等电点-

结肠癌转移关联基因1(MACC1)重组蛋白应用SDS-PAGE; WB; ELISA; IP

Metastasis Metastasis Metastasis Metastasis Associated Associated Associated Associated In Colon Cancer 1 (MACC1) (MACC1) (MACC1) (MACC1)

Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)

Instruction Instruction Instruction Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

6th Edition (Revised in March, 2013)

[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]

Residues: Residues: Residues: Residues: Gln522-Met784 (Accession # Q6ZN28),

with two N-terminal Tags, His-tag and GST-tag. Host: E. coli

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm. Nucleus.

Purity: Purity: Purity: Purity: >95%

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 8.5

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 57.2kDa

Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

结肠癌转移关联基因1(MACC1)重组蛋白[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

结肠癌转移关联基因1(MACC1)重组蛋白[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its

sequence is listed below.

MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL

DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD

VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD

GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQ

GPLGSEF- QKKEEIKSA PLSPKILVKY PTFQDKTLNF SNYGVTLKAV LRQSKIDYFL

EYFKGDTIAL LGEGKVKAIG QSKVKEWYVG VLRGKIGLVH CKNVKVISKE QVMFMSDSVF

TTRNLLEQIV LPLKKLTYIY SVVLTLVSEK VYDWKVLADV LGYSHLSLED FDQIQADKES

EKVSYVIKKL KEDCHTERNT RKFLYELIVA LLKMDCQELV ARLIQEAAVL TSAVKLGKGW

RELAEKLVRL TKQQMEAYEI PHRGNTGDVA VEMM