供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 50ug/100ug/250ug |
货号: | KL047Ca01 |
CAS号: |
肝细胞生长因子(HGF)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位n/a
预测分子量30.0kDa
实际分子量-(差异分析请参阅说明书)
片段与标签Val495~Ser730 with N-terminal His Tag
缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
性状冻干粉
纯度> 95%
肝细胞生长因子(HGF)重组蛋白等电点-
应用SDS-PAGE; WB; ELISA; IP
序列
用法
PBS或其它。
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降
Recombinant Hepatocyte Growth Factor (HGF)
Organism Species: Canis familiaris; Canine (Dog)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PROPERTIES ]
Residues: Val495~Ser730
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q867B7
Host: E. coli
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 8.8
Predicted Molecular Mass: 30.0kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
肝细胞生长因子(HGF)重组蛋白[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
肝细胞生长因子(HGF)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
VVNGIP TRTNVGWMVS LKYRNKHICG GSLIKESWIL TARQCFPSRN RDLKDYEAWL
GIHDVHGKGD EKRKQVLNVS QLVYGPEGSD LVLLKLARPA ILDDFVSTID LPNYGCTIPE
KTTCSVYGWG YTGSINFDGL LRVAHLYIMG NEKCSQYHQG KVTLNESEIC AGAENIVSGP
CEGDYGGPLV CEQHKMRMVL GVIVPGRGCA IPNRPGIFVR VAYYAKWIHK IILTYKIQQS存储,损失率低于5%。
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