供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 50ug/100ug/250ug |
货号: | KL142Hu01 |
CAS号: |
细胞间粘附分子5(ICAM5)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位n/a
预测分子量60.0kDa
实际分子量-(差异分析请参阅说明书)
片段与标签Arg409~Pro674 (Accession # Q9UMF0) with N-terminal His Tag
缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
性状冻干粉
纯度> 95%
等电点-
细胞间粘附分子5(ICAM5)重组蛋白应用SDS-PAGE; WB; ELISA; IP
Intercellular Adhesion Molecule 5 (ICAM5)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC
PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PROPERTIES ]
Residues: Arg409~Pro674 (Accession #
Q9UMF0),
with two N-terminal Tags, His-tag and
GST-tag. Host: E. coli
Subcellular Location: Membrane; Single-
pass
type I membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form
in PBS,
pH7.4, containing 5% sucrose, 0.01%
sarcosyl.
Predicted isoelectric point: 5.7
Predicted Molecular Mass: 60.0kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays
to be determined by the end user.)
细胞间粘附分子5(ICAM5)重组蛋白[ USAGE ]
Reconstitute in sterile PBS, pH7.2-
pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw
cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is
described by the loss rate of the target
protein. The loss rate was determined by
accelerated thermal degradation test,
that is, incubate the protein at 37oC for
48h, and no obvious degradation and
precipitation were observed. (Referring
from China Biological Products Standard,
which was calculated by the Arrhenius
equation.) The loss of this protein is
less
than 5% within the expiration date under
appropriate storage condition.
细胞间粘附分子5(ICAM5)重组蛋白[ SEQUENCES ]
The target protein is fused with two N-
terminal Tags, His-tag and GST-tag, its
sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE
HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA
EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH
PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
KRIEAIPQID KYLKSSKYIA WPLQGWQATF
GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AAAKFERQHM DSPDLGTLEV
LFQGPLGSEF - RL DDSDCPRSWT
WP EG PE Q T L R CE A RG NP E P S VH CA
RS D G GA VL A L G L L GP V TRA LS G TY R
C
KAANDQGEAV KDVTLTVEYA PALDSVGCPE
RITWLEGTEA SLSCVAHGVP PPDVICVRSG
ELGAVIEGLLRVAREHAGTY RCEATNPRGS
AAKNVAVTVE YGPRFEEPSC PSNWTWVEGS
G R L F S C E V D G KP Q P S V K C V G SG
G AT E G V L L P L A P P D P S P R AP R I
P RV L A P
GIYVCNATNRHGSVAKTVVV SAESPPEMDE STCP
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