供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 50ug/100ug/250ug |
货号: | KL243Hu01 |
CAS号: |
对氧磷酶1(PON1)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位n/a
预测分子量20.8kDa
实际分子量-(差异分析请参阅说明书)
片段与标签Gln35~Val206 (Accession # P27169) with N-terminal His Tag
缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
性状冻干粉
纯度> 95%
等电点-
对氧磷酶1(PON1)重组蛋白应用SDS-PAGE; WB; ELISA; IP
Recombinant Paraoxonase 1 (PON1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PROPERTIES ]
Residues: Gln35~Val206 (Accession # P27169),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Secreted, extracellular
space. Purity: >98%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 5.1
Predicted Molecular Mass: 20.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
对氧磷酶1(PON1)重组蛋白[ USAGE ]
Reconstitute in ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- QPVELP NCNLVKGIET GSEDLEILPN GLAFISSGLK YPGIKSFNPN
SPGKILLMDL NEEDPTVLEL GITGSKFDVS SFNPHGISTF TDEDNAMYLL VVNHPDAKST
VELFKFQEEE KSLLHLKTIR HKLLPNLNDI VAVGPEHFYG TNDHYFLDPY LQSWEMYLGL
AWSYVV
对氧磷酶1(PON1)重组蛋白[ REFERENCES ]
1. Hassett C., et al. (1991) Biochemistry 30:10141-10149. 2. Adkins S., et al. (1993) Am. J. Hum. Genet. 52:598-608. 3. Furlong C.E., et al. (1993) Chem. Biol. Interact. 87:35-48. 4. Wang K.K., et al. (1997) Cell Res. 7:79-90.
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