供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 50ug/100ug/250ug |
货号: | KL253Hu01 |
CAS号: |
麦芽糖酶糖化酶(MGA)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位Apical cell membrane; Single-pass type II membrane protei
预测分子量22.0kDa
实际分子量-(差异分析请参阅说明书)
片段与标签Leu213~Asn392 (Accession # O43451) with N-terminal His Tag
缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
性状冻干粉
纯度> 95%
麦芽糖酶糖化酶(MGA)重组蛋白等电点6.2
应用SDS-PAGE; WB; ELISA; IP.
Recombinant Maltase Glucoamylase, Intestinal (MGA)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PROPERTIES ]
Residues: Leu213~Asn392 (Accession # O43451),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Apical cell membrane;
Single-pass type II membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 6.2
Predicted Molecular Mass: 22.0kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
麦芽糖酶糖化酶(MGA)重组蛋白[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-LTYQVEI SRQPFSIKVT RRSNNRVLFD SSIGPLLFAD QFLQLSTRLP
ST NVY G L GE H VH Q QY R H DM N WK TW PI F NR D TT P N G N G T N L YGA Q T F F L C L
EDASGLSFGV FLMNSNAMEV VLQPAPAITY RTIGGILDFY VFLGNTPEQV VQEYLELIGR
PALPSYWALG FHLSRYEYGT LDN
麦芽糖酶糖化酶(MGA)重组蛋白[ REFERENCES ]
1. Nichols B.L., et al. (1998) J. Biol. Chem. 273:3076-3081. 2. Hillier L.W., et al. (2003) Nature 424:157-164. 3. The MGC Project Team. (2004) Genome Res. 14:2121-2127. 4. Naim H.Y., et al. (1988) J. Biol. Chem. 263:19709-19717.
相关产品
关注
拨打电话
留言咨询