供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 50ug/100ug/250ug |
货号: | KL297Hu01 |
CAS号: |
核糖核酸酶A(RNASE1)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU per 1μg (determined by the LAL method).
亚细胞定位分泌
预测分子量16.1kDa
实际分子量18kDa(差异分析请参阅说明书)
片段与标签Lys29~Thr156 with N-terminal His Tag
缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
性状冻干粉
纯度> 90%
等电点8.8
核糖核酸酶A(RNASE1)重组蛋白应用SDS-PAGE; WB; ELISA; IP
Recombinant Ribonuclease A (RNASE1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Lys29~Thr156
Tags: N-terminal His-Tag
Tissue Specificity: Pancreas, Testis, Placenta. Subcellular Location: Secreted. Purity: >92%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Traits: Freeze-dried powder
Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01%
sarcosyl and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;
Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 8.8
Predicted Molecular Mass: 16.1kDa
Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions.
核糖核酸酶A(RNASE1)重组蛋白[ USAGE ]
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ SEQUENCE ]
核糖核酸酶A(RNASE1)重组蛋白[ IDENTIFICATION ]
Figure 1. SDS-PAGE
相关产品
关注
拨打电话
留言咨询