供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 50ug/100ug/250ug |
货号: | KL539Hu01 |
CAS号: |
髓鞘碱性蛋白(MBP)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位细胞膜, 细胞质
预测分子量38.8kDa
实际分子量39kDa(差异分析请参阅说明书)
片段与标签Val220~Met301 with N-terminal His and GST Tag
缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
性状冻干粉
纯度> 97%
等电点9.1
髓鞘碱性蛋白(MBP)重组蛋白应用SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling
Recombinant Myelin Basic Protein (MBP)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
髓鞘碱性蛋白(MBP)重组蛋白[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Val220~Met301
Tags: Two N-terminal Tags, His-tag and GST-tag
Tissue Specificity: Spleen, Lung, Skin. Subcellular Location: Myelin membrane; Peripheral membrane protein;
Cytoplasmic side. Purity: >98%
Traits: Freeze-dried powder
Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01%
sarcosyl and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive
Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 9.1
Predicted Molecular Mass: 38.8kDa
Accurate Molecular Mass: 39kDa as determined by SDS-PAGE reducing conditions. [ USAGE ]
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
髓鞘碱性蛋白(MBP)重组蛋白[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. SDS-PAGE
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