供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 50ug/100ug/250ug |
货号: | KL548Ca01 |
CAS号: |
细胞间粘附分子1(ICAM1)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位n/a
预测分子量85.9kDa
实际分子量-(差异分析请参阅说明书)
片段与标签Gly25~Pro531 with N-terminal His Tag
缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
性状冻干粉
纯度> 90%
细胞间粘附分子1(ICAM1)重组蛋白等电点-
应用SDS-PAGE; WB; ELISA; IP
RPA548Ca01 100μgRecombinant Intercellular Adhesion Molecule 1 (ICAM1)
Organism Species: Canis familiaris; Canine (Dog)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PROPERTIES ]
Residues: Gly25~Pro531
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: F1PB95
Host: E. coli
Subcellular Location: Membrane.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 5.5
Predicted Molecular Mass: 85.9kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
细胞间粘附分子1(ICAM1)重组蛋白[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
细胞间粘附分子1(ICAM1)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
GGAQTS VDPAEAIIPR GGSVQVNCST SCNQTSIFGL ETLLTKKELN SGDNWVLFEL
TDVQEDSKLI CFSNCHEQTM APMHLTVYWF PERVELAPLP RWQPVGENLT MTCQVAGGAP
RTNLTVVLLR GEEELSRQPA VGEPAEVTFT VAVGREDHLA NFSCRTDLDL RHRGLGLFQN
SSAPRQLQTF VLPETPPRLA TPPIVEVGTQ WSVDCTLDGV FPASEAQVHL ALAEERLHST
VLYKKDSLLA TANVKANPED EGTQQLWCEV TLGDENRRWQ ENVTFYSFPA PNLTLSEPEV
SEWTTVTVEC EAPAGVVVSL SGLPSGLAVP RAQFQLNASA ADNRRSFSCS AALEVAGHML
QK NQT RE LHV LYGP R LDQRD CPGNWTWEEG SHQT LKCQAW GNPVPE LK CH
RKGDDALLPI GDLRPVKREV AGTYLCQARS PRGEITREVV INVIYHQNNI LIIILVTTIV
ILGTVSVAAY LYNRQRKIQK YKLQKAQEAA AMKLNTPATP P
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