供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 50ug/100ug/250ug |
货号: | KL655Ra01 |
CAS号: |
钙/钙调蛋白依赖性蛋白激酶Ⅱγ(CAMK2γ)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位n/a
预测分子量36.7kDa
实际分子量-(差异分析请参阅说明书)
片段与标签Tyr14~Lys301 with N-terminal His Tag
缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
性状冻干粉
纯度> 95%
等电点-
钙/钙调蛋白依赖性蛋白激酶Ⅱγ(CAMK2γ)重组蛋白应用SDS-PAGE; WB; ELISA; IP
Recombinant Calcium/Calmodulin Dependent
Protein Kinase II Gamma (CAMK2g)
Organism Species: Rattus norvegicus (Rat)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
钙/钙调蛋白依赖性蛋白激酶Ⅱγ(CAMK2γ)重组蛋白[ PROPERTIES ]
Residues: Tyr14~Lys301
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P11730
Host: E. coli
Subcellular Location: Sarcoplasmic reticulum
membrane, Peripheral membrane protein,
Cytoplasmic side Inferred.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 8.1
Predicted Molecular Mass: 36.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
钙/钙调蛋白依赖性蛋白激酶Ⅱγ(CAMK2γ)重组蛋白[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
YQLFEEL GKGAFSVVRR CVKKTSTQEY AAKIINTKKL SARDHQKLER EARICRLLKH
PNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIHQI LESVNHIHQH
DIVHRDLKPE NLLLASKCKG AAVKLADFGL AIEVQGEQQA WFGFAGTPGY LSPEVLRKDP
YGKPVDIWAC GVILYILLVG YPPFWDEDQH KLYQQIKAGA YDFPSPEWDT VTPEAKNLIN
QMLTINPAKR ITADQALKHP WVCQRSTVAS MMHRQETVEC LRKFNARRKL K
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