供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 50ug/100ug/250ug |
货号: | KL718Hu01 |
CAS号: |
β-位淀粉样前体蛋白裂解酶1(βACE1)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位n/a
预测分子量59.1kDa
实际分子量-(差异分析请参阅说明书)
片段与标签Phe170~Thr428 with N-terminal His Tag
缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
性状冻干粉
纯度> 90%
等电点-
β-位淀粉样前体蛋白裂解酶1(βACE1)重组蛋白应用SDS-PAGE; WB; ELISA; IP.
Recombinant Beta-Site APP Cleaving Enzyme 1 (bACE1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PROPERTIES ]
Residues: Phe170~Thr428
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: P56817
Host: E. coli
Subcellular Location: Membrane; Single-pass type I
membrane protein. Golgi apparatus, trans-Golgi
network. Endoplasmic reticulum. Endosome. Cell
surface. Cytoplasmic vesicle membrane.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 5.2
Predicted Molecular Mass: 59.1kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
β-位淀粉样前体蛋白裂解酶1(βACE1)重组蛋白[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
β-位淀粉样前体蛋白裂解酶1(βACE1)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
F INGSNWEGIL GLAYAEIARP DDSLEPFFDS LVKQTHVPNL FSLQLCGAGF PLNQSEVLAS
VGGSMIIGGI DHSLYTGSLW YTPIRREWYY EVIIVRVEIN GQDLKMDCKE YNYDKSIVDS
GTTNLRLPKK VFEAAVKSIK AASSTEKFPD GFWLGEQLVC WQAGTTPWNI FPVISLYLMG
EVTNQSFRIT ILPQQYLRPV EDVATSQDDC YKFAISQSST GTVMGAVIME GFYVVFDRAR
KRIGFAVSAC HVHDEFRT
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