转甲状腺素蛋白(TTR)重组蛋白

转甲状腺素蛋白(TTR)重组蛋白

来源原核表达

宿主E.coli

内毒素水平<1.0EU/μg(LAL法测定)

亚细胞定位n/a

预测分子量15kDa

实际分子量-(差异分析请参阅说明书)

片段与标签Ser22~Ala147 (Accession # O46375) with N-terminal His Tag

缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）

性状冻干粉

纯度> 95%

等电点-

转甲状腺素蛋白(TTR)重组蛋白应用SDS-PAGE; WB; ELISA; IP

Recombinant Prostaglandin D2 Synthase, Brain (PGD2S)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PROPERTIES ]

Residues: Pro32~Gln190 (Accession # P41222),

with two N-terminal Tags, His-tag and T7-tag.

Host: E. coli

Subcellular Location: Rough endoplasmic reticulum.

Nucleus membrane. Golgi apparatus. Cytoplasm,

perinuclear region. Secreted.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 8.9

Predicted Molecular Mass: 21.6kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

转甲状腺素蛋白(TTR)重组蛋白[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its

sequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- PNFQQDKFL GRWFSAGLAS

NSSWLREKKA ALSMCKSVVA PATDGGLNLT STFLRKNQCE TRTMLLQPAG SLGSYSYRSP

HWGSTYSVSV VETDYDQYAL LYSQGSKGPG EDFRMATLYS RPA726Bo01 100μg

Transthyretin (TTR)

Organism Species: Bos taurus; Bovine (Cattle)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

转甲状腺素蛋白(TTR)重组蛋白[ PROPERTIES ]

Residues: Ser22~Ala147 (Accession # O46375),

with N-terminal His-Tag.

Host: E. coli

Subcellular Location: Secreted.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 6.3

Predicted Molecular Mass: 15.0kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF-SVGAGEPKC PLMVKVLDAV RGSPAANVGV KVFKKAADET

WEPFASGKTS ESGELHGLTT EDKFVEGLYK VELDTKSYWK SLGISPFHEF AEVVFTANDS

GPRHYTIAAL LSPYSYSTTA LVSSPKAEKFTAFCKAQ

GFTEDTIVFL PQTDKCMTEQ