供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 50ug/100ug/250ug |
货号: | KL861Hu01 |
CAS号: |
补体成分3(C3)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位n/a
预测分子量42.4kDa
实际分子量-(差异分析请参阅说明书)
片段与标签Leu1000~Glu1326 (Accession # P01024) with N-terminal His Tag
缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
性状冻干粉
纯度> 95%
等电点-
补体成分3(C3)重组蛋白应用SDS-PAGE; WB; ELISA; IP
Recombinant Complement Component 3 (C3)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PROPERTIES ]
Residues: Leu1000~Glu1326
Tags: Two N-terminal Tags, His-tag and S-tag
Accession: P01024
Host: E. coli
Subcellular Location: Secreted. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 10mM
PBS, pH7.4, containing 1mM DTT, 5% trehalose,
0.01% sarcosyl and preservative.
Predicted isoelectric point: 6.0
Predicted Molecular Mass: 42.4kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
补体成分3(C3)重组蛋白[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
补体成分3(C3)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
L KHLIVTPSGC GEQNMIGMTP TVIAVHYLDE TEQWEKFGLE KRQGALELIK KGYTQQLAFR
QPSSAFAAFV KRAPSTWLTA YVVKVFSLAV NLIAIDSQVL CGAVKWLILE KQKPDGVFQE
DAPVIHQEMI GGLRNNNEKD MALTAFVLIS LQEAKDICEE QVNSLPGSIT KAGDFLEANY
MNLQRSYTVA IAGYALAQMG RLKGPLLNKF LTTAKDKNRW EDPGKQLYNV EATSYALLAL
LQ L K D F DF VP PVV RW L NEQ R YYG G GYGST Q AT FMVFQA LA QY QK DA P D H Q
ELNLDVSLQL PSRSSKITHR IHWESASLLR SEETKE
[ REFERENCES ]
1. de Bruijn M.H.L., Fey G.H. (1985) Proc. Natl. Acad. Sci. U.S.A. 82:708-712. 2. Baldo A., et al. (1993) J. Clin. Invest. 92:1543-1547. 3. Oxvig C., et al. (1995) J. Biol. Chem. 270:13645-13651.
4. Isaac L., Isenman D.E. (1992) J. Biol. Chem. 267:10062-10069.
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