毒蕈碱型胆碱受体M1(CHRM1)重组蛋白

毒蕈碱型胆碱受体M1(CHRM1)重组蛋白

来源原核表达

宿主E.coli

内毒素水平<1.0EU/μg(LAL法测定)

亚细胞定位n/a

预测分子量18.3kDa

实际分子量-(差异分析请参阅说明书)

片段与标签Arg210~Ala363 (Accession # P08482) with N-terminal His Tag

缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）

性状冻干粉

纯度> 95%

等电点-

毒蕈碱型胆碱受体M1(CHRM1)重组蛋白应用SDS-PAGE; WB; ELISA; IP

Cholinergic Cholinergic Cholinergic Cholinergic Receptor, Receptor, Receptor, Receptor, Muscarinic Muscarinic Muscarinic Muscarinic 1 (CHRM1) (CHRM1) (CHRM1) (CHRM1)

Organism: Organism: Organism: Organism: Rattus norvegicus norvegicus norvegicus norvegicus (Rat)

Instruction Instruction Instruction Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

5th Edition (Revised in January, 2013)

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Cholinergic Receptor, Muscarinic 1

Synonyms: Synonyms: Synonyms: Synonyms: CHRM1, Chrm-1

Species: Species: Species: Species: Rat

Size: 100μg

Source: Source: Source: Source: Escherichia coli-derived

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cell membrane; Multi-pass

membrane protein. Cell junction, synapse,

postsynaptic cell membrane.

毒蕈碱型胆碱受体M1(CHRM1)重组蛋白[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]

Residues: Residues: Residues: Residues: Arg210~Ala363 (Accession # P08482),

with N-terminal His-Tag. Grade & Purity: Purity: Purity: Purity: >95%, 24kDa as determined by

SDS-PAGE reducing conditions.

Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS, pH

7.4, containing 0.1% Sarcosyl, 5% sucrose.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by

the LAL method).

Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 18.3kDa

Rat CHRM1 kDa

94

66.2

45

33

26

20

14.4

15% SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE

SDS-PAGE is a technique that separates proteins based on size. However, the actual band size

observed may differ from the predicted; the common factors may include three terms:

1. Alternative splicing (splice variants).

2. 2. The composition of amino acids may give the protein the different relative charge.

3. Polymerization of the target protein.

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 9.1

毒蕈碱型胆碱受体M1(CHRM1)重组蛋白[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF-R IYRETENRAR ELAALQGSET PGKGGGSSSS SERSQPGAEG

SPESPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV

VIKMPMVDSE AQAPTKQPPK SSPNTVKRPT KKGRDRGGKG QKPRGKEQLA

KRKTFSLVKE KKA

[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1. Takamori M., et al. (2007) Eur. J. Neurol. 14:1230-1235.

2. Maeda Y., et al. (2006) Am. J. Respir. Crit. Care Med. 174:1119-1124.

3. Santafe M.M., et al. (2003) Eur. J. Neurosci. 17:119-127.

4. Anagnostaras S.G., et al. (2003) Nat. Neurosci. 6:51-58.