粘蛋白4(MUC4)重组蛋白

粘蛋白4(MUC4)重组蛋白

来源原核表达

宿主E.coli

内毒素水平<1.0EU/μg(LAL法测定)

亚细胞定位Cell membrane; Single-pass membrane protein, Secrete

预测分子量19.3kDa

实际分子量-(差异分析请参阅说明书)

片段与标签Pro1154~Glu1309 (Accession # Q99102) with N-terminal His Tag

缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）

性状冻干粉

纯度> 95%

等电点6.8

粘蛋白4(MUC4)重组蛋白应用SDS-PAGE; WB; ELISA; IP

Recombinant Mucin 4 (MUC4)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PROPERTIES ]

Residues: Pro1154~Glu1309 (Accession # Q99102),

with N-terminal His-Tag.

Host: E. coli

Subcellular Location: Cell membrane;

Single-pass membrane protein, Secreted. Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 6.8

Predicted Molecular Mass: 19.3kDa

Accurate Molecular Mass: 22kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

粘蛋白4(MUC4)重组蛋白[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

粘蛋白4(MUC4)重组蛋白[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGS- PFWDDAD FSTGRGTTFY QEYETFYGEH SLLVQQAESW IRKMTNNGGY

KA RWA L K V TW VN A HAY PA QW TL G S N T Y Q AI LS T D G S RS YA LF LY QS G GM Q

WDVAQRSGNP VLMGFSSGDG YFE NSP LMSQ PVWERYRPDR FL NS NSG LQG

LQFYRLHRE

[ REFERENCES ]

1. Siezen C.L., et al. (2009) Pediatr. Infect. Dis. J. 28:333-335. 2. Zhang J., et al. (2006) Tissue Cell 38:271-275. 3. Picariello G., et al. (2008) Proteomics 8:3833-3847. 4. Karg A., et al. (2006) Pathol. Res. Pract. 202:577-583.