供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 50ug/100ug/250ug |
货号: | KL072Hu01 |
CAS号: |
泛素连接酶E3A(UBE3A)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位细胞核
预测分子量32.0kDa
实际分子量40kDa(差异分析请参阅说明书)
片段与标签Ser158~Leu407 with N-terminal His Tag
缓冲液成份20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
性状冻干粉
纯度> 90%
等电点6.1
泛素连接酶E3A(UBE3A)重组蛋白应用SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling.
Recombinant Ubiquitin Protein Ligase E3A (UBE3A)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Ser158~Leu407
Tags: N-terminal His-Tag
Tissue Specificity: Brain, Testis. Subcellular Location: Nucleus. Purity: >92%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification;
Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.1
Predicted Molecular Mass: 32.0kDa
Accurate Molecular Mass: 40kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as
follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form泛素连接酶E3A(UBE3A)重组蛋白. 5. Polymerization of the target protein: Dimerization, multimerization etc. [ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0
mg/mL. Do not vortex. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. 泛素连接酶E3A(UBE3A)重组蛋白[ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. Gene Sequencing (Extract)
Figure 2. SDS-PAGE
相关产品
关注
拨打电话
留言咨询