| 供货周期： | 现货 |
| 品牌： | 康朗生物 |
| 规格： | 50ug/100ug/250ug |
| 货号： | KL091Hu01 |
| CAS号： |
肝肾骨碱性磷酸酶(ALPL)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位Cell membrane; Lipid-anchor, GPI-anch
预测分子量39.5kDa
实际分子量-(差异分析请参阅说明书)
片段与标签Leu18~Arg335 (Accession # P05186) with N-terminal His Tag
缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
性状冻干粉
纯度> 95%
等电点7.0
肝肾骨碱性磷酸酶(ALPL)重组蛋白应用SDS-PAGE; WB; ELISA; IP.
Recombinant Alkaline Phosphatase, Liver/Bone/Kidney (ALPL)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PROPERTIES ]
Residues: Leu18~Arg335 (Accession # P05186),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Cell membrane;
Lipid-anchor, GPI-anchor.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 7.0
Predicted Molecular Mass: 39.5kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
肝肾骨碱性磷酸酶(ALPL)重组蛋白[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
肝肾骨碱性磷酸酶(ALPL)重组蛋白[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF - LVP EKEKDPKYWR
DQAQETLKYA LELQKLNTNV AKNVIMFLGD GMGVSTVTAA RILKGQLHHN PGEETRLEMD
KFPFVALSKT YNTNAQVPDS AGTATAYLCG VKANEGTVGV SAATERSRCN TTQGNEVTSI
LRWAKDAGKS VGIVTTTRVN HATPSAAYAH SADRDWYSDN EMPPEALSQG CKDIAYQLMH
NIRDIDVIMG GGRKYMYPKN KTDVEYESDE KARGTRLDGL DLVDTWKSFK PRYKHSHFIW
NRTELLTLDP HNVDYLLGLF EPGDMQYELN RNNVTDPSLS EMVVVAIQIL RKNPKGFFLL
VEGGR
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