供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 50ug/100ug/250ug |
货号: | KL099Hu01 |
CAS号: |
CD8a分子(CD8a)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位细胞膜, 分泌
预测分子量20.6kDa
实际分子量24/28kDa(差异分析请参阅说明书)
片段与标签Ser22~Val198 with N-terminal His Tag
缓冲液成份20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
性状冻干粉
纯度> 90%
等电点8.8
CD8a分子(CD8a)重组蛋白应用SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling
Recombinant Cluster Of Differentiation 8a (CD8a)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
CD8a分子(CD8a)重组蛋白[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Ser22~Val198
Tags: N-terminal His-Tag
Subcellular Location: Cell membrane; Single-pass type I membrane protein. Secreted. Purity: >92%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive
Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 8.8
Predicted Molecular Mass: 20.6kDa
Accurate Molecular Mass: 24&28kDa as determined by SDS-PAGE reducing
conditions. Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as
follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. [ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0
mg/mL. Do not vortex. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ SEQUENCE ]
CD8a分子(CD8a)重组蛋白[ IDENTIFICATION ]
Figure 1. Gene Sequencing (Extract)
Figure 2. SDS-PAGE
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