供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 50ug/100ug/250ug |
货号: | KL212Hu01 |
CAS号: |
嗜铬蛋白A(CHGA)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位分泌, 细胞质
预测分子量16.4kDa
实际分子量20kDa(差异分析请参阅说明书)
片段与标签Gln15~Gly148 with N-terminal His Tag
缓冲液成份20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
性状冻干粉
纯度> 97%
等电点5.1
嗜铬蛋白A(CHGA)重组蛋白应用SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling
Recombinant Chromogranin A (CHGA)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Gln15~Gly148
Tags: N-terminal His-Tag
Tissue Specificity: Liver, Stomach, Brain, Pancreas. Subcellular Location: Cytoplasmic vesicle, secretory vesicle lumen.. Purity: >98%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;
Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.1
Predicted Molecular Mass: 16.4kDa
Accurate Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions. [ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0
mg/mL. Do not vortex.
嗜铬蛋白A(CHGA)重组蛋白[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ SEQUENCE ]
嗜铬蛋白A(CHGA)重组蛋白[ IDENTIFICATION ]
Figure 1. SDS-PAGE
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