| 供货周期： | 现货 |
| 品牌： | 康朗生物 |
| 规格： | 50ug/100ug/250ug |
| 货号： | KL336Hu01 |
| CAS号： |
保护素(CD59)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位Cell membrane; Lipid-anchor, GPI-anchor. Secret
预测分子量14.7kDa
实际分子量-(差异分析请参阅说明书)
片段与标签Leu26~Asn102 (Accession # P13987) with N-terminal His Tag
缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
性状冻干粉
纯度> 95%
等电点5.5
保护素(CD59)重组蛋白应用SDS-PAGE; WB; ELISA; IP.
Recombinant Protectin (CD59)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
保护素(CD59)重组蛋白[ PROPERTIES ]
Residues: Leu26~Asn102 (Accession # P13987),
with two two N-terminal Tags, His-tag and S-tag. Host: E. coli
Subcellular Location: Cell membrane;
Lipid-anchor, GPI-anchor. Secreted. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 5.5
Predicted Molecular Mass: 14.7kDa
Accurate Molecular Mass: 19kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
保护素(CD59)重组蛋白[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two two N-terminal Tags, His-tag and S-tag, its
sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- LQCYN
CPNPTADCKT AVNCSSDFDA CLITKAGLQV YNKCWKFEHC NFNDVTTRLR ENELTYYCCK
KDLCNFNEQL EN
[ REFERENCES ]
1. Davies A., et al. (1989) J. Exp. Med. 170:637-654. 2. Sugita Y., et al. (1989) J. Biochem. 106:555-557. 3. Sawada R., et al. (1990) DNA Cell Biol. 9:213-220. 4. Tone M., et al. (1992) J. Mol. Biol. 227:971-976.
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