供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 50ug/100ug/250ug |
货号: | KL360Hu01 |
CAS号: |
酸性神经鞘磷脂酶(ASM)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位n/a
预测分子量33.9kDa
实际分子量30kDa(差异分析请参阅说明书)
片段与标签His319~Pro579 (Accession # P17405) with N-terminal His Tag
缓冲液成份20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
性状冻干粉
纯度> 95%
等电点6.6
酸性神经鞘磷脂酶(ASM)重组蛋白应用SDS-PAGE; WB; ELISA; IP
Recombinant Acid Sphingomyelinase (ASM)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PROPERTIES ]
Residues: His319~Pro579
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P17405
Host: E. coli
Subcellular Location: Lysosome.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 6.6
Predicted Molecular Mass: 33.9kDa
Accurate Molecular Mass: 30kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
酸性神经鞘磷脂酶(ASM)重组蛋白[ USAGE ]
Reconstitute in ddH2O. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
酸性神经鞘磷脂酶(ASM)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
HE STPVNSFPPP FIEGNHSSRW LYEAMAKAWE PWLPAEALRT LRIGGFYALS PYPGLRLISL
NMNFCSRENF WLLINSTDPA GQLQWLVGEL QAAEDRGDKV HIIGHIPPGH CLKSWSWNYY
RIVARYENTL AAQFFGHTHV DEFEVFYDEE TLSRPLAVAF LAPSATTYIG LNPGYRVYQI
DGNYSGSSHV VLDHETYILN LTQANIPGAI PHWQLLYRAR ETYGLPNTLP TAWHNLVYRM
RGDMQLFQTF WFLYHKGHP
[ REFERENCES ]
1. Schuchman E.H., et al. (1991) J. Biol. Chem. 266:8531-8539. 2. Newrzella D., Stoffel W. (1992) Biol. Chem. Hoppe-Seyler 373:1233-1238. 3. Schuchman E.H., et al. (1992) Genomics 12:197-205. 4. Ida H., et al. (1993) J. Biochem. 114:15-20.
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