唾液淀粉酶α1(AMY1)重组蛋白

唾液淀粉酶α1(AMY1)重组蛋白

来源原核表达

宿主E.coli

内毒素水平<1.0EU/μg(LAL法测定)

亚细胞定位Secreted

预测分子量57.2kDa

实际分子量-(差异分析请参阅说明书)

片段与标签Ala15~Leu511 (Accession # P04745) with N-terminal His Tag

缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）

性状冻干粉

纯度> 95%

等电点6.5

唾液淀粉酶α1(AMY1)重组蛋白应用SDS-PAGE; WB; ELISA; IP

Recombinant Amylase Alpha 1, Salivary (AMY1)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PROPERTIES ]

Residues: Ala15~Leu511 (Accession # P04745),

with N-terminal His-Tag.

Host: E. coli

Subcellular Location: Secreted.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 6.5

Predicted Molecular Mass: 57.2kDa

Accurate Molecular Mass: 43kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

唾液淀粉酶α1(AMY1)重组蛋白[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHH HHHHSG S -AQYSS NTQQ GRTSIV H L FE WRWV DIA LE C ERY LAPKG FG

GVQVS PP NE N VAI HNP F RPW WE RY QP VSYK LC T RS GNE DE FR NMVT RC NN

VGVRIYVDAV INHMCGNAVS AGTSSTCGSY FNPGSRDFPA VPYSGWDFND GKCKTGSGDI

ENYNDATQVR DCRLSGLLDL ALGKDYVRSK IAEYMNHLID IGVAGFRIDA SKHMWPGDIK

AILDKLHNLN SNWFPEGSKP FIYQEVIDLG GEPIKSSDYF GNGRVTEFKY GAKLGTVIRK

WNGEKMSYLK NWGEGWGFMP SDRA LVFV DN HDNQRGHGAG GASILTFWDA

RLYKMAVGFM LA HPYGF T RV MSSY RWP RYF ENGK DV NDWV GPP NDNGVTK

EVTI NP DT T C GNDWV CE HRW RQIR NMV NF R NVV DGQPF T N WY DNGS NQVA

FGRGNRGFIV FNNDDWTFSL TLQTGLPAGT YCDVISGDKI NGNCTGIKIY VSDDGKAHFS

ISNSAEDPFI AIHAESKL

唾液淀粉酶α1(AMY1)重组蛋白[ REFERENCES ]

1. Baltova S.D. (2005) Folia Med (Plovdiv) 47:37-41.

2. Ramasubbu N., et al. (2004) Arch. Biochem. Biophys. 421:115-124.

3. Ramasubbu N., et al. (2004) Eur. J. Biochem. 271:2517-2529.

4. Ramachandran P., et al. (2006) J. Proteome Res. 5:1493-1503.