供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 50ug/100ug/250ug |
货号: | KL482Hu01 |
CAS号: |
唾液淀粉酶α1(AMY1)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU per 1μg
亚细胞定位分泌
预测分子量45.0kDa
实际分子量45.0kDa(差异分析请参阅说明书)
片段与标签Gln19~Ile376 (Accession # Q06828) with N-terminal His Tag
缓冲液成份100mM NaHCO3, 500mM NaCl缓冲液(pH8.3,含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
性状冻干粉
纯度> 95%
等电点6.1
唾液淀粉酶α1(AMY1)重组蛋白应用SDS-PAGE; WB; ELISA; IP
Recombinant Fibromodulin (FMOD)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PROPERTIES ]
Residues: Gln19~Ile376
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q06828
Host: E. coli
Subcellular Location: Secreted, extracellular
space, extracellular matrix.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 100mM
NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA,
1mM DTT, 0.01% sarcosyl, 5% trehalose, and
preservative.
Predicted isoelectric point: 6.1
Predicted Molecular Mass: 45.0kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
唾液淀粉酶α1(AMY1)重组蛋白[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
唾液淀粉酶α1(AMY1)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
QY EDDPHWWFHY LRSQQSTYYD PYDPYPYETY EPYPYGVDEG PAYTYGSPSP
PDPRDCPQEC DCPPNFPTAM YCDNRNLKYL PFVPSRMKYV YFQNNQITSI QEGVFDNATG
LLWIALHGNQ ITSDKVGRKV FSKLRHLERL YLDHNNLTRM PGPLPRSLRE LHLDHNQISR
VPNNALEGLE NLTALYLQHN EIQEVGSSMR GLRSLILLDL SYNHLRKVPD GLPSALEQLY
MEHNNVYTVP DSYFRGAPKL LYVRLSHNSL TNNGLASNTF NSSSLLELDL SYNQLQKIPP
VNTNLENLYL QGNRINEFSI SSFCTVVDVV NFSKLQVLRL DGNEIKRSAM PADAPLCLRL
ASLIEI
[ REFERENCES ]
1. Antonsson P., et al. (1993) Biochim. Biophys. Acta 1174:204-206. 2. Hildebrand A., et al. (1994) Biochem. J. 302:527-534. 3. Mayr C., et al. (2005) Blood 105:1566-1573. 4. Mikaelsson E., et al. (2005) Blood 105:4828-4835.
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