表面活性物质关联蛋白B(SPB)重组蛋白

表面活性物质关联蛋白B(SPB)重组蛋白

来源原核表达

宿主E.coli

内毒素水平<1.0EU/μg(LAL法测定)

亚细胞定位分泌, 细胞外基质

预测分子量51.1kDa

实际分子量51kDa(差异分析请参阅说明书)

片段与标签Phe201~Leu381 with N-terminal His and GST Tag

缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）

性状冻干粉

纯度> 90%

等电点6.8

表面活性物质关联蛋白B(SPB)重组蛋白应用SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling

Recombinant Coagulation Factor XIII B Polypeptide (F13B)

Organism Species: Mus musculus (Mouse)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

表面活性物质关联蛋白B(SPB)重组蛋白[ PROPERTIES ]

Residues: Ala21~Gln208

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q07968

Host: E. coli

Subcellular Location: Secreted.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 7.6

Predicted Molecular Mass: 25.0kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

表面活性物质关联蛋白B(SPB)重组蛋白[ SEQUENCES ]

The sequence of the target protein is listed below.

AEEKQCDFPT VENGRIAQYY YTFKSFYFPM SVDKKLSFFC LAGYATESGK QEEQIRCTAE

GWSP NP RCYK KC LKP D L RNG YVS NDKV LYK LQE RMSYG CS SGYK T TGGK D

EEVVHCLSAG WSSQPSCRKE QETCLAPELE HGNYSTTQRT FP91620Hu01 P91620Hu01 P91620Hu01 P91620Hu01

C4 Binding Binding Binding Binding Protein Protein Protein Protein Alpha (C4BPa) (C4BPa) (C4BPa) (C4BPa)

Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)

Instruction Instruction Instruction Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

4th Edition (Revised in August, 2012)

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: C4 Binding Protein Alpha

Synonyms Synonyms Synonyms Synonyms: C4BPa, C4BP

Species: Species: Species: Species: Human

Size: 100μg

Source: Escherichia coli-derived

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Secreted.

[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]

Residues Residues Residues Residues: Gln548~Leu597 (Accession # P04003), with N-terminal His-Tag.

Grade & Purity: Purity: Purity: Purity: >95%, 7 kDa as determined by SDS-PAGE reducing conditions.

Formulation Formulation Formulation Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 7.3 kDa

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 6.0

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was

determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious

degradation and precipitation were observed. (Referring from China Biological Products Standard, which was

calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under

appropriate storage condition.

[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]

The target protein is fused with N-terminal His-tag, its sequence is listed below.

MGHHHHHHSGSEF-QVL TGKRLMQCLP NPEDVKMALE VYKLSLEIEQ LELQRDSARQ STLDKELVKDIVAYT CTAGYYTTTRPB622Hu01 10μg

Recombinant Surfactant Associated Protein B (SPB)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

11th Edition (Revised in May, 2016)

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Phe201~Leu381

Tags: Two N-terminal Tags, His-tag and GST-tag

Tissue Specificity: Lung, Brain. Subcellular Location: Secreted, extracellular space, surface film. Purity: >92%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification;

Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.8

Predicted Molecular Mass: 51.1kDa

Accurate Molecular Mass: 51kDa as determined by SDS-PAGE reducing conditions. [ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0

mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate

was determined by accelerated thermal degradation test, that is, incubate the

protein at 37

oC for 48h, and no obvious degradation and precipitation were

observed.The loss rate is less than 5% within the expiration date under

appropriate storage condition. [ SEQUENCE ]

[ IDENTIFICATION ]

Figure 1. SDS-PAGE

KQTGEAECQA NGWSLTPQ