新霉素磷酸转移酶2抗体

Quantity size:

0.2ml

Concentration:

1mg/ml 新霉素磷酸转移酶2抗体  Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Background:

Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin? G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation,新霉素磷酸转移酶2抗体 which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.

新霉素磷酸转移酶2抗体Also known as:

Aminoglycoside 3' phosphotransferase; APH(3') II; APH(3')II; aphA; EC 2.7.1.95; Kan; Kanamycin kinase type II; Kanamycin kinase, type VIII; kanamycin resistance gene; Neo; Neomycin kanamycin phosphotransferase type II; Neomycin kanamycin phosphotransferase type VIII; Neomycin phosphotransferase II; Neomycine phosphotransferase; NPT II; NPTII.

Specificity:

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Rabbit Polyclonal IgG,新霉素磷酸转移酶2抗体 affinity purified by Protein A.

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Reacts with: Escherichia coli,Reacts with bacteria..

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Immunogen: KLH conjugated synthetic peptide derived from human Neomycin Phosphotransferase 2.

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新霉素磷酸转移酶2抗体Predicted Molecular Weight: 32kDa.

Storage:

Shipped at 4℃, Store at -20℃ (Avoid repeated freeze/thaw cycles).

Application:

WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500   
Not yet tested in other applications. 
Optimal working dilutions must be determined by the end user.

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