磷酸化增殖细胞核抗原抗体

Quantity size:

0.2ml

Concentration:

1mg/ml 磷酸化增殖细胞核抗原抗体  Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Background:

Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) 磷酸化增殖细胞核抗原抗体for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure.

磷酸化增殖细胞核抗原抗体Also known as:

PCNA (phospho Y211); p-PCNA (phospho Y211); PCNA(Tyr211); PCNA; Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; Pcna/cyclin; PCNAR; Polymerase delta accessory protein; Proliferating Cell Nuclear Antigen; PCNA_HUMAN.

Specificity:

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Rabbit Polyclonal IgG, 磷酸化增殖细胞核抗原抗体affinity purified by Protein A.

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Reacts with: Human, Mouse, Rat, Chicken, Cow, Rabbit, Sheep, .

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Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PCNA around the phosphorylation site of Tyr211.

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磷酸化增殖细胞核抗原抗体Predicted Molecular Weight: 29kDa.

Storage:

Shipped at 4℃, Store at -20℃ (Avoid repeated freeze/thaw cycles).

Application:

WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  Flow-Cyt=1:30-300  IF=1:100-500   
Not yet tested in other applications. 
Optimal working dilutions must be determined by the end user.

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