磷酸化丝氨酸/苏氨酸抗体

Quantity size:

0.1ml

Concentration:

1mg/ml  磷酸化丝氨酸/苏氨酸抗体 Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Background:

A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases, but also those that are recognized by phosphorylation-dependent binding proteins like 14-3-3. 磷酸化丝氨酸/苏氨酸抗体These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine surrounded by phenylalanine or tyrosine. For example, Akt, an important kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site surrounded by phenylalanine and tyrosine. RSK1, p70 S6 K,磷酸化丝氨酸/苏氨酸抗体 and certain PKC isoforms also contain a similar consensus phosphorylation site. Phosphorylation of these sites is required for kinase activity. The Phospho-(Ser/Thr) Phe Antibody is a powerful tool for discovery of new proteins containing this important regulatory motif.

磷酸化丝氨酸/苏氨酸抗体Also known as:

phospho-Ser/Phospho-Thr; pS/pT; p-S/p-T; pSER/pThr; p-SER/p-Thr; p-Sp-T.

Specificity:

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Rabbit Polyclonal IgG, affinity purified by Protein A.

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Reacts with: Phosphoserine/threonine.

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Immunogen: KLH conjugated synthesised phosphopeptide contain Phosphoserine and 磷酸化丝氨酸/苏氨酸抗体Phosphothreonine.

Storage:

Shipped at 4℃, Store at -20℃ (Avoid repeated freeze/thaw cycles).

Application:

ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500   
Not yet tested in other applications. 
Optimal working dilutions must be determined by the end user.

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