mTOR相关调控蛋白抗体

Quantity size:

0.2ml

Concentration:

1mg/ml mTOR相关调控蛋白抗体  Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Background:

mTOR controls cell growth, in part by regulating p70 S6 kinase alpha (p70alpha) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor is a 150 kDa mTOR binding protein that also binds 4EBP1 and p70alpha. The binding of Raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinasemTOR相关调控蛋白抗体 activity toward p70alpha. Rapamycin or amino acid withdrawal increases, whereas insulin strongly inhibits, the recovery of 4EBP1 and raptor on 7-methyl-GTP Sepharose. Partial inhibition of raptor expression by RNA interference (RNAi) reduces mTOR-catalyzed 4EBP1 phosphorylation in vitro. RNAi of C. elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo.

mTOR相关调控蛋白抗体Also known as:

p150 target of rapamycin (TOR) scaffold protein containing WD repeats; P150 target of rapamycin (TOR)-scaffold protein; Regulatory Associated Protein of mTOR; KIAA1303; RPTOR_HUMAN; Regulatory-associated protein of mTOR; p150 target of rapamycin (TOR)-scaffold protein.

Specificity:

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Rabbit Polyclonal IgG,mTOR相关调控蛋白抗体 affinity purified by Protein A.

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Reacts with: Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, .

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Immunogen: KLH conjugated synthetic peptide derived from human Raptor.

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mTOR相关调控蛋白抗体Predicted Molecular Weight: 147kDa.

Storage:

Shipped at 4℃, Store at -20℃ (Avoid repeated freeze/thaw cycles).

Application:

WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500   
Not yet tested in other applications. 
Optimal working dilutions must be determined by the end user.

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