磷酸化波形蛋白抗体

Quantity size:

0.1ml

Concentration:

1mg/ml磷酸化波形蛋白抗体   Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Background:

Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate磷酸化波形蛋白抗体 filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin.磷酸化波形蛋白抗体 Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

磷酸化波形蛋白抗体Also known as:

Vimentin (phospho S56); p-Vimentin (phospho S56); FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.

Specificity:

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Rabbit Polyclonal IgG, affinity purified by Protein A.

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Reacts with: Human, Mouse, Rat, Dog, Pig, Cow, Sheep, Guinea Pig, .

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Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Vimentin around the phosphorylation site of Ser56.

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磷酸化波形蛋白抗体Predicted Molecular Weight: 51kDa.

Storage:

Shipped at 4℃, Store at -20℃ (Avoid repeated freeze/thaw cycles).

Application:

WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  Flow-Cyt=1:50-500  IF=1:100-500   
Not yet tested in other applications. 
Optimal working dilutions must be determined by the end user

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