Anti-JMJD6抗体

Anti-JMJD6抗体

Product Properties

Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)

200058Anti-JMJD6(N-term) Mouse mAbMouse mAbHumanWB,ICC62

Sensitivity  This antibody detects endogenous levels of JMJD6(N-terminus) and does not cross-react with related proteins.

Immunogen  Purified recombinant human JMJD6(N-terminus) fragments expressed in E.coli.

Host  Mouse

Clone Number  3G5-G5-H8

Antibody type  Monoclonal antibody

Anti-JMJD6抗体

Purified method  Affinity purified

Isotype  IgG1

Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol

Dilution

Anti-JMJD6抗体WB~~1:1000

ICC~~1:200

Western blot detection of JMJD6(N-terminus) in Hela and HL-60 lysates using JMJD6(N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 46KDa.Observed band size: 62KDa.

Immunocytochemistry of HeLa cells using anti-JMJD6(N-terminus) mouse mAb diluted 1:200.

Anti-JMJD6抗体Summary  This gene encodes a nuclear protein with a JmjC domain. JmjC domain-containing proteins are predicted to function as protein hydroxylases or histone demethylases. This protein was first identified as a putative phosphatidylserine receptor involved in phagocytosis of apoptotic cells; however, subsequent studies have indicated that it does not directly function in the clearance of apoptotic cells, and questioned whether it is a true phosphatidylserine receptor. Multiple transcript variants encoding different isoforms have been found for this gene.

UniPort summary Function  Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses.

Alternate names  Apoptotic cell clearance receptor;Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6;Histone arginine demethylase JMJD6;JmjC domain-containing protein 6;JMJD 6;JMJD6;JMJD6_HUMAN;Jumonji domain containing 6;Jumonji domain-containing protein 6;KIAA0585;Lysyl-hydroxylase JMJD6;Peptide-lysine 5-dioxygenase JMJD6;Phosphatidylserine receptor;Protein PTDSR;PSR;PTDSR 1;PTDSR;PTDSR1.

Gene_symbol  PSR; PTDSR; PTDSR1

UniProt Number  Q6NYC1

GeneBank ID  NM_001081461

Gene ID  23210

Subcellular location  Nucleus