供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 100ul/50ul/25ul |
货号: | KL201082-2A9 |
CAS号: |
Anti-Histone H2A.X抗体
Product Properties
Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)
201082-2A9Anti-Histone H2A.X(Phospho-Ser139) Mouse mAbMouse mAbHuman,MouseWB,IF15
Sensitivity This antibody detects endogenous levels of H2A.X only when phosphorylated at serine 139.
Immunogen Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.
Anti-Histone H2A.X抗体Host Mouse
Clone Number 2A9-B5
Antibody type Monoclonal antibody
Purified method Affinity purified
Isotype IgG2a
Anti-Histone H2A.X抗体Formulation Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Dilution
WB~~1:2000
ICC~~1:400
Anti-Histone H2A.X抗体Other~~1:400
Application Images-expand |
Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.
Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X
Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in A549(upper,untreated or Hydroxyurea-treated) and Hela(lower,untreated or Hydroxyurea-treated) using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).
Western blot analysis of extracts from untreated or Hydroxyurea-treated Hela and A549 cells, using Histone H2A.X(Phospho-Ser139) mouse mAb (1:1000 diluted) (upper) or β-Actin Mouse mAb (200068-8F10) (lower).Predicted band size:15KDa.Observed band size:15KDa.
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