Anti-Histone H2A.X抗体

参考价:¥1190
供货周期: 现货
品牌: 康朗生物
规格: 100ul/50ul/25ul
货号: KL201082-2A9
CAS号:
上海康朗生物科技有限公司
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产品详情

Anti-Histone H2A.X抗体

Product Properties

Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)

201082-2A9Anti-Histone H2A.X(Phospho-Ser139) Mouse mAbMouse mAbHuman,MouseWB,IF15

Sensitivity  This antibody detects endogenous levels of H2A.X only when phosphorylated at serine 139.


Immunogen  Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.


Anti-Histone H2A.X抗体Host  Mouse


Clone Number  2A9-B5


Antibody type  Monoclonal antibody


Purified method  Affinity purified


Isotype  IgG2a


Anti-Histone H2A.X抗体Formulation  Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.


Dilution

WB~~1:2000

ICC~~1:400

Anti-Histone H2A.X抗体Other~~1:400

Application Images

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Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.


Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X


Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in A549(upper,untreated or Hydroxyurea-treated) and Hela(lower,untreated or Hydroxyurea-treated) using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).


Western blot analysis of extracts from untreated or Hydroxyurea-treated Hela and A549 cells, using Histone H2A.X(Phospho-Ser139) mouse mAb (1:1000 diluted) (upper) or β-Actin Mouse mAb (200068-8F10) (lower).Predicted band size:15KDa.Observed band size:15KDa.



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