| 供货周期： | 现货 |
| 品牌： | 康朗生物 |
| 规格： | 100ul/50ul/25ul |
| 货号： | KL310012 |
| CAS号： |
Anti-NFκB-p65抗体
Product Properties
Cat CodeProduct NameClonality
Cross reactivityApplication
MW(kDa)
310012Anti-NFκB-p65(Phospho-Thr254)
Anti-NFκB-p65抗体Rabbit pAbRabbit pAb
Human,Mouse,RatWB,IHC,ICC65
Sensitivity The antibody detects
endogenous level of NF-κB p65 only when
phosphorylated at threonine 254.
Anti-NFκB-p65抗体Immunogen Peptide sequence around
phosphorylation site of threonine 254 (F
-R-T(p)-P-P) derived from Human NFκB-
p65.
Host Rabbit
Clone Number
Anti-NFκB-p65抗体Antibody type Polyclonal antibody
Purified method
Isotype IgG
Formulation Supplied at 1.0mg/mL in
phosphate buffered saline (without Mg2+
and Ca2+), pH 7.4, 150mM NaCl, 0.02%
sodium azide and 50% glycerol.
Dilution
WB~~1:500-1:1000
ICC~~1:100-1:200
Application Images-expand |
Western blot analysis of extracts from 3T3 cells untreated or treated with TNF-α using NFκB-p65 (Phospho-Thr254) Antibody .
Immunofluorescence staining of methanol-fixed Hela using NFκB-p65(Phospho-Thr254) Antibody .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Thr254) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot detection of NFkB-p65(Phospho-Ser254) in 3T3 cells untreated or treated with Alkaline phosphatase using NFkB-p65(Phospho-Ser254) Rabbit pAb (dilution 1:500, upper) or β-Actin Mouse mAb (200068-8F10, lower).Predicted band size:65kDa.Observed band size:65kDa.
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